Effects of mineral trioxide aggregate on the expression of TNF α and IL 1 in human stem cells from apical papilla

Effects of mineral trioxide aggregate on the expression of TNF α and IL 1 in human stem cells from apical papilla

摘要

Objective To determine the effects of mineral trioxide aggregate （MTA） on the expression of TNF α and IL 1 in human stem cells from apical papilla （SCAPs）.Methods SCAPs were isolated by magnetic activated cell sorting （MACS） and cultured in α MEM medium containing 2mg/mL MTA for 1 day and 3 days.Enzyme linked immunosorbent assay （ELISA） was applied to examine the TNF α and IL 1 in the culture supernatant of SCAPs.Quantitative RT PCR was used to determine the gene expression of TNF α, IL 1α, and IL 1β in SCAPs.Results The expression levels of TNF α and IL 1 at both protein expression and mRNA transcription were inhibited （P<001） when the cells were treated with MTA.Conclusion MTA can inhibit the expression of early stage inflammatory cytokines and relieve the inflammation via reducing the secretion of inflammatory cytokines.