呼伦贝尔草原不同环境介质中特定抗生素耐药性基因的分布与演变特征

Distribution and evolution of certain antibiotic resistance genes in different environmental media in Hulunbuir Grassland

摘要

采用荧光定量PCR、16S rRNA测序和网络分析等方法，研究2019—2021年夏季3种环境介质样品（雨水、表层土壤和PM_2.5）中特定的抗生素耐药基因（ARGs）。结果显示：呼伦贝尔草原3种环境介质均存在ARGs污染，并且土壤中丰度最高；与ARGs显著相关的细菌菌属有助于相关抗性基因在多种环境介质中分布传播并增强ARGs的潜在暴露风险。

Abstract

We measured the relative abundance of antibiotic resistance genes （ARGs） related to the treatment of brucellosis by quantitative real-time polymerase chain reaction （PCR）， characterized the bacterial composition by 16S rRNA gene sequencing， and analysed the relationships between ARGs and bacteria by network analyses in multiple environmental media samples （rainwater， topsoil and PM_2.5） collected in the summers from 2019 to 2021. The three environmental media of Hulunbuir Grassland were contaminated with some degree of ARGs with the highest ARG abundance in the topsoil. Significant relationships were observed between ARGs and bacteria， thereby promoting the spread of antibiotic resistance. Extensive distribution of ARG carrying bacteria in atmospheric particles， rainwater， and soil may also increase potential ARG exposure risks. These findings are valuable in understanding the ARG pollution levels and distribution characteristics in three environmental media， which can aid to design and implement policies to curb ARGs in local areas.

关键词

抗生素耐药性基因（ARGs） / 分布 / 丰度 / 演变 / 多种环境介质 / 呼伦贝尔草原

Key words

ARGs / distribution / abundance / evolution / multiple environmental media / Hulunbuir Grassland