金沙江石斛根部木霉属内生真菌的鉴定及耐旱性研究

Identification and Drought Tolerance Study of Endophytic Trichoderma in the Roots of Dendrobium wangliangii

摘要

本研究以濒危兰科植物金沙江石斛（Dendrobium wangliangii）根部5株内生真菌为对象，通过分子鉴定，5株内生真菌均为木霉属（Trichoderma）菌株，利用PEG-6000模拟干旱胁迫，测定不同浓度梯度下菌株的光密度值（OD₆₀₀），并结合隶属函数法评价其耐旱性差异。结果表明：5株菌株耐旱性呈现多样性，其中MA-R-3-4（Trichoderma viridescens）在15% PEG-6000模拟干旱胁迫下生长最佳（OD=0.697±0.096），MA-R-3-21（Trichoderma viride）与R-3-4（Trichoderma atroviride）在25% PEG-6000模拟干旱胁迫下OD值达到峰值，R-3-3（Trichoderma scalesiae）与R-3-1（Trichoderma atroviride）在40% PEG-6000高渗条件下仍保持较高活性。本研究初步证实金沙江石斛根部木霉属内生真菌的耐旱潜力，为后续共生机制研究及物种保育提供理论基础。

Abstract

This study focused on the five endophytic fungi isolated from the roots of the endangered orchid Dendrobium wangliangii. Molecular identification revealed that all five endophytic fungal strains belonged to the genus Trichoderma. Simulated drought stress was induced using PEG-6000, and the optical density values (OD₆₀₀) of the strains were measured under different concentration gradients. Their drought tolerance differences were evaluated using the membership function method. The results indicated diversity in the drought tolerance among the five strains: Strain MA-R-3-4 (Trichoderma viridescens) exhibited optimal growth (OD=0.697±0.096) under 15% PEG-6000 simulated drought stress; the OD values of strains MA-R-3-21 (Trichoderma viride) and R-3-4 (Trichoderma atroviride) peaked under 25% PEG-6000 stress; strains R-3-3 (Trichoderma scalesiae) and R-3-1 (Trichoderma atroviride) maintained relatively high activity even under high osmotic stress with 40% PEG-6000. This study preliminarily confirms the drought tolerance potential of endophytic Trichoderma fungi from the roots of Dendrobium wangliangii, providing a theoretical basis for subsequent research on symbiotic mechanisms and species conservation.

关键词

金沙江石斛 / 木霉属 / 内生真菌 / PEG-6000 / 耐旱性

Key words

Dendrobium wangliangii / Trichoderma / endophytic fungi / PEG-6000 / drought tolerance

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基金资助

国家自然科学基金项目(32160100)

大学生创新训练项目(202111393088)