脂肪酶类产品对伏马毒素B1脱毒效果的研究

Study on the detoxification effect of lipase products on fumonisin B1

摘要

伏马毒素(FBs)性质稳定，常规生产加工很难破坏其结构，导致在农产品中残留严重。众多脱毒方法中，酶法脱毒因其安全高效、对环境影响小等优点而备受青睐。为了探索脂肪酶类产品对FB1的脱毒效果，本研究评估了3种脂肪酶类产品的脱毒性能，3种脂肪酶类产品在最优条件下对1000 ng/mL的FB1的脱毒率均接近100%，进一步在玉米粉、花生粕、酒糟蛋白饲料(DDGS)3种饲料原料以及胃肠液的场景应用中评估，最高脱毒率分别为(88.00±0.19)%和(90.42±0.07)%。通过液相色谱-质谱联用法(LC-MS)发现脂肪酶产品作用方式为“降解-吸附”双重机制，降解产物为C₂₂H₄₇NO₅。对FB1脱毒效果的研究，为实现规模化脱除FB1的应用提供了重要的理论支持，具有潜在的应用前景。

Abstract

Fumonisins (FBs) have stable properties and are difficult to break down in their structure through conventional production and processing, resulting in severe residues in agricultural products. Among various detoxification methods, enzymatic detoxification is highly favored due to its safety, efficiency, and minimal environmental impact. In order to explore the detoxification effect of lipase products on fumonisin B1 (FB1), this study evaluated the detoxification performance of three kinds of lipase products. Under the optimal conditions, the detoxification rate of the three kinds of lipase products on 1000 ng/mL FB1 was close to 100%. Further, the highest detoxification rate was (88.00±0.19)% and (90.42±0.07)% respectively in the scenario application of three kinds of feed raw materials, namely, jade Rice noodles, peanut meal, and distillers dried grains with solids (DDGS), as well as gastrointestinal juice. Through liquid chromatography-mass spectrometry (LC-MS), it was found that the action mode of lipase product is a dual mechanism of "degradation adsorption", and the degradation product is C₂₂H₄₇NO₅. The study on the detoxification effect of FB1 provides important theoretical support for the large-scale application of FB1 removal and has potential application prospects.

关键词

伏马毒素B1 / 脂肪酶产品 / 降解－吸附机制 / 饲料应用研究

Key words

fumonisin B1 / lipase products / degradation-adsorption mechanism / feed application research

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基金资助

山东省重点研发计划(2022CXGC020801)