基于不同SERS基底对蒺藜提取物拉曼光谱信号强度的影响研究

A Study on the Influence of Different SERS Substrates on the Raman Spectral Signal Intensity of Tribulus Extract

摘要

目的 本研究结合表面增强拉曼光谱（SERS）与蒺藜提取方法，探究不同SERS基底对其提取物拉曼信号强度的影响。以蒺藜为对象，采用30 min、80 W的最佳超声条件提取去氢骆驼蓬碱、芦丁，通过氧化还原法制备AuNPs，结合SERS技术分析目标化合物光谱特征，对比AuNPs、锌、铝等6种基底的增强效果。结果 成功制备出增强效果最佳的SERS基底，可为蒺藜快速分析及中药材质量评价提供研究基础。

Abstract

Objective This study combines Surface-Enhanced Raman Spectroscopy (SERS) with Tribulus extract preparation methods to investigate the influence of different SERS substrates on the Raman signal intensity of the extract. Taking Tribulus as the object, dehydro-N-Oxymatrine and Rutin were extracted under optimal ultrasonic conditions of 30 minutes and 80 W. AuNPs were prepared by redox method, and the spectral characteristics of target compounds were analyzed using SERS technology. The enhancement effects of six kinds of substrates including AuNPs, zinc, and aluminum were compared. Results The SERS substrate with the best enhancement effect was successfully prepared, which can provide a research basis for rapid analysis of Tribulus and quality evaluation of Chinese medicinal materials.

关键词

蒺藜 / 去氢骆驼峰碱 / 芦丁 / SERS

Key words

Tribulus / Dehydro-N-Oxymatrine / Rutin / SERS

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