金纳米棒在近红外二区光声成像引导肿瘤光热治疗中的应用（英文）

王如意; 邢洋; 范博; 张瑞平

doi:10.13451/j.sxu.ns.2024107

山西大学学报(自然科学版) ›› 2025, Vol. 48 ›› Issue (04) : 793 -805. DOI: 10.13451/j.sxu.ns.2024107

化学

金纳米棒在近红外二区光声成像引导肿瘤光热治疗中的应用（英文）

王如意 ¹ ,
邢洋 ¹ ,
范博 ¹ ,
张瑞平 ²

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Gold Nanorods for Photoacoustic Imaging-guided Tumor Photothermal Therapy in the Second Near-infrared Region

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摘要

近红外二（the Second Near-infrared II, NIR-II）区光声（Photoacoustic,PA）成像引导光热治疗（Photothermal Therapy,PTT）具有增强穿透深度和提高信号强度等优势而备受关注。该方法可实现实时肿瘤诊断和原位治疗，在临床应用中具有显著优势。因此，迫切需要开发在NIR-II区具有临床转化潜力的光热材料。本文采用种子生长法制备了在NIR-II区具有强吸收的金纳米棒（Gold Nanorods,GNRs），用于PA成像引导下的PTT研究。GNRs的光热转换效率高达36.68%，光热稳定性好。修饰后的聚乙二醇-金纳米棒（Polyethylene Glycol-Gold Nanorods,GNRsPEG），其细胞毒性较低，对小鼠乳腺癌细胞（4T1）细胞具有光热消融作用。建立原位乳腺癌模型，GNRs-PEG在4T1荷瘤小鼠体内具有良好光热治疗作用。此外，体内实验结果证明了GNRs-PEG通过PA成像引导的NIR-II区PTT消除肿瘤具有良好的治疗效果。本研究合成的GNRs-PEG作为NIR-II PA造影剂，在成像引导下的癌症治疗中具有广泛的应用前景。

Abstract

Photoacoustic (PA) imaging-guided photothermal therapy (PTT) in the second near-infrared (NIR-II) region has garnered increasing attention due to its inherent advantages, such as enhanced penetration depth and heightened signal intensity. This approach enables real-time tumor diagnosis and in-situ treatment simultaneously, offering significant advantages in clinical translation. Nevertheless, there is an urgent need for the development of photothermal agents with clinical translational potential in the NIR-II region. Here, we successfully synthesized gold nanorods (GNRs) exhibiting strong absorption in the NIR-II region, which enables precise PA imaging-guided PTT. GNRs has a high photothermal conversion efficiency of 36.68% and good photothermal stability. After being modified with polyethylene glycol (PEG), in vitro experiments showed that GNRs-PEG had ignorable cytotoxicity and showed efficient ablation of 4T1 cells. An orthotopic breast cancer model was established to study the in vivo anti-tumor efficacy of GNRs-PEG on 4T1 tumor-bearing mice. The results showed that GNRs-PEG had an enhanced tumor inhibitory effect. Additionally, in vivo PA imaging can be used to track the dynamic distribution of GNRs-PEG at the tumor site. Furthermore, in vivo results demonstrated the high effectiveness of GNRs-PEG for tumor elimination through PA imaging-guided PTT in the NIR-II region. In conclusion, the GNRs-PEG synthesized in this work hold broad applications as NIR-II PA contrast agents for imaging-guided cancer therapy.