梨杂种后代矮生与乔生株根系内生微生物群落多样性比较

Comparison of Root Endophytic Microbial Community Diversity Between Dwarf and Arborescent Progenies of Pear Hybrids

摘要

为探究梨树根系内生微生物的群落组成及其与寄主树型的相关性，该研究以7年生乔生和矮生梨树为研究对象，利用16S rRNA和ITS高通量测序技术对梨树杂种群体根系内生微生物的群落结构进行了分析。结果表明，矮生梨树群体根系内生细菌群落与树体高度、树干周长和直径呈显著相关，而乔生梨树群体在两者间则没有呈显著相关。矮生树群体根系内生细菌、真菌的共现网络比乔生树群体更为复杂。其中，矮生树群体主要富集了拟杆菌门、蓝细菌门等类群的细菌，乔生树群体主要富集了变形菌门等类群的细菌；矮生树群体主要富集了子囊菌门等类群的真菌，乔生树群体主要富集了担子菌门等类群的真菌。矮生与乔生梨树群体塑造了其各自独特的细菌与真菌微生物群落。

Abstract

To investigate the composition of root endophytic microbial communities in pear trees and their correlation with host tree types, this study analyzes the root endophytic microbial community structure in 7-year-old arborescent and dwarf pear hybrid populations, using 16S rRNA and ITS high-throughput sequencing technologies. The results demonstrate that the root endophytic bacterial community in the dwarf pear population exhibits significant correlations with tree height, trunk circumference, and trunk diameter, whereas the arborescent pear population shows no such significant correlations. The co-occurrence networks of endophytic bacteria and fungi in the dwarf population are more complex than those in the arborescent population. Specifically, the dwarf population predominantly enriches bacterial taxa such as Bacteroidota and Cyanobacteria, while the arborescent population enriches bacterial taxa such as Proteobacteria. For fungi, the dwarf population mainly enriches fungal taxa such as Ascomycota, whereas the arborescent population enriches fungal taxa such as Basidiomycota. Both dwarf and arborescent pear populations shape their own distinct bacterial and fungal microbial communities.

关键词

梨树 / 矮生 / 乔生 / 杂种群体 / 微生物群落

Key words

Pear trees / dwarf / arborescent / hybrid population / microbial community

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基金资助

山东省良种工程(2019LZGC008)