番茄 SlGlyI 基因家族鉴定及生物信息学分析

Identification and Bioinformatics Analysis of Tomato SlGlyI Gene Family

摘要

GlyI（glyoxalase Ⅰ）基因负责调控乙二醛酶I的表达，相关研究表明乙二醛酶I在植物抵抗逆境胁迫中发挥重要作用。为了鉴定番茄 GlyI基因家族，初步探究 SlGlyI基因的生物学功能，本研究基于拟南芥、烟草、马铃薯 GlyI基因，在番茄（ Solanum lycopersicum L .）中筛选出8个 GlyI基因。根据它们在染色体上的位置，命名为 SlGlyI-1到 SlGlyI-8，并对其理化性质和组织表达模式等方面进行了系统分析，明确了番茄响应盐胁迫过程中 GlyI 基因表达模式。结果表明：1）共线性分析显示拟南芥和番茄 GlyI基因家族间存在4对基因共线性关系；2）对番茄、拟南芥、马铃薯GlyI蛋白构建系统进化树，依据拟南芥的分类情况将 SlGlyI基因家族分为3个亚族；3） SlGlyI基因家族的保守结构域包括VOC-like结构域和PLN02367 superfamily结构域；4）在 SlGlyI基因家族的启动子区域发现了27个与激素响应、光响应、生长发育和逆境响应等相关的顺式作用元件；5）组织表达模式分析显示， SlGlyI基因家族成员在根、茎、叶中皆有表达，除 SlGlyI-4外其余均在叶中表达量最多；6）在盐胁迫下， SlGlyI-1、 SlGlyI-2、 SlGlyI-4、 SlGlyI-5、 SlGlyI-8 基因表达上调； SlGlyI-3 和 SlGlyI-7基因表达下调； SlGlyI-6基因表达不受影响。综上，探究番茄 SlGlyI基因家族的生物学功能，可为 SlGlyI的功能和分子机理研究提供理论基础。

Abstract

GlyI (glyoxalase Ⅰ) gene is responsible for regulating the expression of glyoxalase I. Related studies have shown that glyoxalase I plays an important role in plant resistance to stress. In order to identify the GlyI gene family in tomato and preliminarily explore the biological functions of SlGlyI genes, this paper screened eight GlyI genes in tomato ( Solanum lycopersicum L.) based on the GlyI genes of Arabidopsis, tobacco and potato. They are named SlGlyI-1 to SlGlyI-8 according to their location on the chromosome, and their physicochemical properties and tissue expression patterns are systematically analyzed, clarifying the expression pattern of GlyI genes in tomato during salt stress response. The results indicate that: 1) Collinearity analysis reveals four pairs of collinearity relationships between the GlyI gene families of Arabidopsis and tomato; 2) A phylogenetic tree is constructed for GlyI proteins from tomato, Arabidopsis, and potato, and the SlGlyI gene family is classified into three subfamilies based on the classification in Arabidopsis; 3) The conserved domains of SlGlyI gene family include VOC-like domain and PLN02367 superfamily domain; 4) In the promoter region of the SlGlyI gene family, 27 cis-acting elements related to hormone response, light response, growth and development, and stress response are found; 5) Expression pattern analysis showed that SlGlyI gene family members are expressed in roots, stems, and leaves, with the highest expression in leaves except SlGlyI-4. 6) Under salt stress, the expression of SlGlyI-1, SlGlyI-2, SlGlyI-4, SlGlyI-5 and SlGlyI-8 genes is up-regulated; SlGlyI-3 and SlGlyI-7 are down-regulated; SlGlyI-6 gene expression remains unaffected. In summary, exploring the biological functions of the SlGlyI gene family in tomato provides a theoretical basis for studying the functional and molecular mechanism of SlGlyI.

关键词

番茄 / GlyI基因家族 / 生物信息学 / 基因表达分析

Key words

Tomato / GlyI gene family / bioinformatics / gene expression analysis

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