根内根孢囊霉和多菌灵对鼓粒期大豆生物量及根际土壤细菌总数的影响

Effects of Rhizophagus intraradices and Carbendazim on Soybean Biomass and Total Rhizosphere Soil Bacterial Count During Pod-Filling Stage

摘要

随着全球大豆需求持续增长，大豆的产量也愈发受到人们关注。根腐病作为典型的土传病害，严重威胁大豆稳产与可持续生产。丛枝菌根真菌可以降低大豆根腐病患病率，从而提高大豆产量。为探索提高大豆产量、促进可持续发展的有效方法，本试验以垦豆3原种大豆为试验材料，通过大田试验研究正茬、迎茬种植方式下根内根孢囊霉（ Rhizophagus intraradices）与多菌灵对鼓粒期大豆生物量以及根际土壤细菌总数的影响。结果表明，同正茬种植相比，迎茬种植会使大豆植株主要生物量与细菌总数下降；正茬与迎茬种植下，接种 R. intraradices的处理组，其株高、茎粗、根长及地上鲜重均高于其他处理组。本研究旨在克服大豆迎茬障碍、增产增效，为可持续种植与病害绿色防控策略提供新途径。

Abstract

As global soybean demand continues to rise, soybean yield has garnered increasing attention. Root rot, a typical soil-borne disease, poses a huge threat to stable and sustainable soybean production. Arbuscular Mycorrhizal Fungi (AMF) can reduce the incidence of soybean root rot, thereby enhancing yield. To explore effective methods for increasing soybean yield and promoting sustainable development, this study utilizes Kandou 3 (original variety) soybean as experimental material. Field trials are conducted to investigate the effects of Rhizophagus intraradices and carbendazim on soybean biomass and total rhizosphere soil bacterial count during the pod-filling stage under rotational cropping and alternative cropping systems. The results show that compared to rotational cropping, alternate cropping reduces primary plant biomass and total bacterial count in soybeans. Under both rotational and alternate cropping systems, the Rhizophagus intraradices-inoculated treatment group exhibits greater plant height, stem diameter, root length, and above-ground fresh weight than other treatment groups. This study aims to overcome obstacles in soybean alternate cropping, enhance yield and production efficiency, and provide a novel approach for sustainable cultivation and green disease control strategies.

关键词

大豆 / 根内根孢囊霉 / 正茬与迎茬 / 生物量 / 多菌灵

Key words

Soybean / Rhizophagus intraradices / rotational cropping and alternate cropping / biomass / carbendazim

参考文献

[1] 高峰, 王剑, 魏同洋, 等. 全球变局下的我国大豆产业安全研究[J]. 中国油脂, 2024, 11(28): 1-10.

[2] 张旭辉. 接种根瘤菌对豌豆重茬障碍的缓解效应研究[D].兰州:甘肃农业大学, 2021.

[3] 张晓聪, 孙小雪, 杨晓燕, 等. 连作北沙参根际土壤化感物质鉴定及其化感效应分析[J]. 中药材, 2025(01): 21-29.

[4] 孙金英, 郭峰, 白银凤, 等. 迎茬对大豆农艺性状及产量的影响[J]. 农村科技, 2025(01): 14-16.

[5] Wang Y, Xu X, Liu T, et al. Analysis of bacterial and fungal communities in continuous-cropping ramie (Boehmeria nivea L.Gaud) fields in different areas in China [J]. Scientific Reports, 2020, 10(1): 3564-3569.

[6] 王子凡, 李燕, 张庆银. 微生物菌剂对设施番茄主要病害及土壤微生物群落的影响[J]. 中国农业科技导报, 2024, 26(06): 102-112.

[7] 田蜜, 陈应龙, 李敏, 等. 丛枝菌根结构与功能研究进展[J]. 应用生态学报, 2013, 24(08): 2369.

[8] 屈明华, 俞元春, 李生, 等. 丛枝菌根真菌对矿质养分活化作用研究进展[J]. 浙江农林大学学报, 2019, 36(02): 394-405.

[9] 汪伯晏, 陈金, 程齐修, 等. 丛枝菌根真菌培养与应用研究进展[J]. 植物研究, 2025, 45(03): 361-370.

[10] Ma X, Luo W, Li J, et al. Arbuscular mycorrhizal fungi increase both concentrations and bioavilability of Zn in wheat (Triticum aestivum L) grain on Zn-spiked soils [J]. Applied Soil Ecology, 2018, 135: 91-97.

[11] Moarrefzadeh N, Khateri H, Abbasi S . Alleviation of Rhizoctonia root rot damages in common bean by some arbuscular mycorrhizal fungi [J]. Journal of Applied Research in Plant Protection, 2023, 12(1): 13-24.

[12] 黄京华, 谭钜发, 揭红科, 等. 丛枝菌根真菌对黄花蒿生长及药效成分的影响[J]. 应用生态学报, 2011, 22(06): 1443-1449.

[13] 杨柳, 姚博翰, 唐依伟. 丛枝菌根真菌与印度梨形孢对枳生长与养分的影响[J]. 现代园艺, 2025, 48(05): 13-15.

[14] 李柄霖, 丁德东, 何静, 等. 丛枝菌根真菌对枸杞生长及抗根腐病的影响[J]. 云南农业大学学报(自然科学版), 2022, 37(04): 547-552.

[15] 接伟光, 林厚泽, 杨冬莹, 等. 根内根孢囊霉对大豆成熟期生物量、根腐病及根际土壤百菌清残留的影响[J]. 江苏农业科学, 2023, 51(14): 153-158.

[16] 谢夏, 杨建兰, 刘新育. 土壤中细菌菌落总数的测定条件优化[J]. 中国农学通报, 2020, 36(11): 92-95.

[17] 聂梦果, 李朋发, 蒋建东. 根际微生物协助植物抵御极端土壤水分胁迫研究进展[J]. 应用与环境生物学报, 2025, 31(09): 1487-1498.

[18] 谢伟, 郝志鹏, 张莘, 等. 丛枝菌根网络介导的植物间信号交流研究进展及展望[J]. 植物生态学报, 2022, 46(05): 493-515.

[19] 陈品璐, 杜霞, 罗雪峰, 等. 蔬菜主要连作病害发生现状、病原特性及趋势分析[J]. 植物医学, 2025, 4(01): 1-11.

[20] 刘坤雨, 刘威, 谷雪菲, 等. 种衣剂和生物菌剂对连作大豆结瘤和生长的影响[J]. 北方农业学报, 2024, 52(06): 31-38.

[21] Russell M, Řezáčová V, Miller K S, et al. Common mycorrhizal networks improve survival and mediate facilitative plant interactions among Andropogon gerardii seedlings under drought stress[J]. Mycorrhiza, 2025, 35(1): 1-14.

[22] Vieira K C, Marascalchi N M, Rozmoš M, et al. Arbuscular mycorrhizal fungal highways-What, how and why? [J]. Soil Biology and Biochemistry, 2025, 202: 109702.

[23] Jia B, Cui X, Zhang Z, et al. Arbuscular mycorrhizal fungi regulate amino acid metabolism,phytohormones and glycolysis pathway to promote the growth of Suaeda salsa under combined Cd and NaCl stresses[J]. Plant Physiology and Biochemistry, 2024, 214: 108921.

[24] 张金波, 王永斌, 谭巍巍, 等. 大豆根际微生物的研究进展[J]. 大豆科学, 2024, 43(04): 471-480.

[25] Huang D, Ma M, Wang Q, et al. Arbuscular mycorrhizal fungi enhanced drought resistance in apple by regulating genes in the MAPK pathway[J]. Plant Physiology and Biochemistry, 2020, 149: 245-255.

[26] Pei Y, Siemann E, Tian B, et al. Root flavonoids are related to enhanced AMF colonization of an invasive tree[J]. AoB Plants, 2020, 12(1): plaa002.

[27] Lin Z, Feng G, D Stéphane . Signal beyond nutrient,fructose,exuded by an arbuscular mycorrhizal fungus triggers phytate mineralization by a phosphate solubilizing bacterium[J]. Isme Journal, 2018, 12(10): 2339-2351.

[28] Prischmann V D A, Tülin Z, Laura A W, et al. Microbial inoculants differentially influence plant growth and biomass allocation in wheat attacked by gall-inducing hessian fly (Diptera: Cecidomyiidae) [J]. Environmental Entomology, 2020, 49(5): 1214-1225.

[29] Jan J, 冯固, 段世龙, 等 . 丛枝菌根真菌菌丝际及其在土壤碳和养分循环中的重要性[J]. 植物研究, 2025, 45(03): 333-336.

[30] 张月秋, 袁野, 邢立国, 等. 多菌灵在我国小麦和水稻上的环境风险评估研究[J]. 现代农药, 2024, 23(06): 66-72.

[31] Qian S, Xu Y, Zhang Y, et al. Effect of AMF inoculation on reducing excessive fertilizer use[J]. Microorganisms, 2024, 12(8): 1550- 1550.

基金资助

黑龙江省自然科学基金联合引导项目(LH2023C087)

黑龙江东方学院科研创新平台建设项目(KYPT202402)

黑龙江省生态环境保护科研项目(HST2022TR001)