超声波辅助提取桔梗皂苷的工艺优化

Optimization of Ultrasound-Assisted Extraction Process for Saponins from Platycodon grandiflorus

摘要

本研究以淄博市产桔梗为原料，通过超声波辅助法优化桔梗皂苷的提取。首先探究四种预处理方式对桔梗皂苷得率与抗氧化活性的影响，进一步分析超声时间、超声功率、乙醇浓度对皂苷提取的影响，采用单因素试验结合响应面法，对提取桔梗皂苷的工艺条件进行系统优化并验证。结果表明，将新鲜桔梗匀浆后，皂苷提取率较高、抗氧化活性较强且工艺更简便；工艺优化试验表明，当乙醇浓度为71%、超声功率259 W、提取时间46 min时，桔梗皂苷得率最高，达9.62%。本研究建立的超声波辅助法进一步优化了桔梗皂苷的提取工艺，为桔梗资源高值化利用提供技术支撑。

Abstract

This study applies the ultrasonic-assisted extraction (UAE) to optimize the extraction of saponins from Platycodon grandiflorus produced in Zibo City. Firstly, the effects of four different pretreatment methods on the yield and antioxidant activity of Platycodon saponins are investigated. Subsequently, the parameters of ultrasonic time, ultrasonic power and ethanol concentration are investigated. A systematic optimization and validation of the extraction process conditions for Platycodon saponins are conducted using single-factor experiments combined with response surface methodology (RSM). The Results demonstrate that homogenization of fresh platycodon grandiflorus yields a relatively higher extraction rate, stronger antioxidant activity and a simplified procedure. The process optimization experiments indicate that the highest saponin yield (9.62%) is achieved under the conditions of 71% ethanol concentration, 259 W ultrasonic power and 46 min extraction time. The UAE established in this study further optimizes the extraction process of saponins, providing a technical foundation for the high-value utilization of platycodon grandiflorus resources.

关键词

桔梗皂苷 / 超声波辅助提取 / 响应面优化 / 单因素试验 / 抗氧化活性

Key words

Platycodon grandiflorus saponins / ultrasound-assisted extraction / response surface optimization / single-factor experiment / antioxidant activity

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基金资助

山东省现代农业产业技术体系中草药体系淄博综合试验站(SDAIT-20-13)