小麦黄花叶病田不同深度土壤致病力分析和病毒分离物鉴定

Pathogenicity Analysis of Wheat Yellow Mosaic Virus-Infested Soil at Different Depths and Identification of WYMV Isolates

摘要

小麦黄花叶病毒（ Wheat yellow mosaic virus， WYMV）是一种重要的小麦土传病毒病害，近年来其发病率有上升趋势。实地调查发现，山东省临沂市费县小麦黄花叶病发生严重。采集病区不同深度（0~10 cm、10~20 cm、20~30 cm、30~40 cm）的土壤进行检测发现，该病区的土壤中含有WYMV，且随着土层的深度增加，病土的致病力逐层下降。对费县病田中的WYMV外壳蛋白（CP）序列进行扩增并构建系统进化树发现，临沂WYMV分离物的CP聚类在一起，且与陕西地区WYMV分离物（SX2 clone 22）进化关系较近。本研究结果表明，小麦黄花叶病田中表层土的致病力最强，且在同一病田中存在两种以上进化关系较近的WYMV分离物，系统进化分析为进一步探究WYMV的演化方向和设计进化保守位点靶标药物奠定了基础。

Abstract

Wheat yellow mosaic virus (WYMV) is a significant soil-borne viral disease of wheat, and its incidence has shown an increasing trend in recent years. Field investigation reveals a severe occurrence of WYMV in Fei County, Linyi City, Shandong Province. Soil samples from different depths (0~10 cm, 10~20 cm, 20~30 cm, 30~40 cm) in the affected area are collected and tested, confirming the presence of WYMV in the soil. Furthermore, the pathogenicity of the infested soil decreases progressively with increasing soil depth. The coat protein (CP) sequences of WYMV isolates from the diseased fields in Fei County are amplified and used to construct a phylogenetic tree. The results show that the CP sequences of WYMV isolates from Linyi cluster together and exhibit a close evolutionary relationship with WYMV isolates from Shaanxi (SX2 clone 22). The results of this study indicate that the topsoil in wheat yellow mosaic disease-infected fields possesses the strongest pathogenicity. Furthermore, more than two evolutionarily related WYMV isolates coexist in the same infected field. The phylogenetic analysis lays a foundation for further exploring the evolutionary direction of WYMV and designing drugs targeting conserved evolutionary sites.

关键词

小麦黄花叶病毒 / WYMV临沂分离物 / 致病力分析 / 系统进化分析

Key words

Wheat yellow mosaic virus / Linyi isolate of WYMV / pathogenicity analysis / phylogenetic analysi

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基金资助

NSFC-国家自然科学基金面上项目(31872638)

山东农业大学德州小麦产业研究院项目(539703018002)