负载布洛芬光交联水凝胶敷料的生物性能及抑菌镇痛性能研究

Study on biological properties, antibacterial and analgesic activities of ibuprofen-loaded photocrosslinked hydrogel wound dressing

摘要

目的: 开发具有良好抑菌性能和生物相容性的多功能水凝胶,用于治疗细菌感染创面,起到快速愈合伤口和止痛的作用。方法: 本文以甲基丙烯酰化明胶和聚丙烯酰胺为原料,搭载硫化铜粒子和布洛芬,采用光交联的方式制备三维多孔结构的复合水凝胶,具有良好的生物相容性和抗菌性能。通过扫描电子显微镜(SEM)、红外光谱仪(FT-IR)、X射线衍射仪(XRD)等仪器对其形貌和结构进行表征,分析聚丙烯酰胺/甲基丙烯酰化明胶载药复合水凝胶的溶胀降解性、力学性能和流变性,同时进行生物体外实验,验证其优异的生物相容性,并探究其抗菌性能。结果: PG-CuS@Ib水凝胶具有高溶胀性能(100%±5%),能有效吸收渗出液;对大肠埃希菌和金黄色葡萄球菌的抗菌测试显示,抑菌率均超过99%;生物相容性测试显示,MC3T1-E1细胞存活率较高,细胞形态舒展,且无明显的溶血现象(<5%)。结论: PG-CuS@Ib多功能复合水凝胶能够满足伤口愈合的关键要求。总体而言,PG-CuS@Ib水凝胶作为伤口敷料具有一定应用前景。

Abstract

Objective: To develop multifunctional hydrogels with excellent antibacterial properties and biocompatibility for the treatment of bacterial infection wounds, which can rapidly heal wounds and relieve pain. Methods: A three-dimensional porous composite hydrogel was prepared by using methacrylated gelatin and polyacrylamide as raw materials and loading copper sulfide particles and ibuprofen through photo-crosslinking. The hydrogel has good biocompatibility and antibacterial properties. The morphology and structure of the hydrogel were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffractometer (XRD), etc. The swelling and degradation properties, mechanical properties and rheological properties of the drug-loaded PAAM/GelMA composite hydrogel were analyzed. Meanwhile, in vitro biological experiments were conducted to verify its excellent biocompatibility and explore its antibacterial properties. Results: The PG-CuS@Ib hydrogel displayed a high swelling performance (100%±5%), which can effectively absorb exudate. The antibacterial tests against Escherichia coli and Staphylococcus aureus showed that the inhibition rates were both over 99%. In the biocompatibility test, the survival rate of MC3T1-E1 cells was high, the cell morphology was spread out, and there was no obvious hemolysis (<5%). Conclusion: The PG-CuS@Ib multifunctional composite hydrogel can meet the key requirements for wound healing. In general, the PG-CuS@Ib hydrogel has promising prospects as a wound dressing.

关键词

硫化铜纳米粒子 / 布洛芬 / 抑菌性 / 镇痛 / 水凝胶

Key words

copper sulfide nanoparticles / ibuprofen / antibacterial activity / relieve pain / hydrogel