姜黄素同轴载药复合纤维膜的质量及功效评价研究

Quality and efficacy evaluation of curcumin-coaxial drug-loaded composite fibrous membranes

摘要

目的: 设计一种具有核壳结构的同轴载药复合纤维膜。采用静电纺丝和喷雾技术制备获得了同轴载药纤维膜,以实现促进伤口愈合和抑菌的效果。方法: 对制备的复合纤维膜进行理化性质表征、质量控制以及体内外功效评价。结果: 观察到同轴纳米纤维具有明显的核壳结构,表现出优异的抑菌效果。通过HE染色、血管内皮生长因子(VEGF)免疫组化证明同轴载药复合纤维膜能够促进胶原合成,加速新生血管生成以及促进伤口愈合。结论: 所制备的同轴载药复合纤维膜各项测定结果均符合要求,可达到促进伤口愈合并抑制细菌的效果,为新型伤口敷料的研发提供了新的思路。

Abstract

Objective: To design a coaxial drug-loaded composite fiber membrane with a core-shell structure. The coaxial drug-loaded fiber membrane was fabricated using electrospinning and electrospraying techniques, achieving satisfactory quality with demonstrated efficacy in promoting wound healing and antibacterial activity. Methods: Physicochemical characterization, quality control, and in vitro and in vivo efficacy evaluation were conducted on the fabricated composite fiber membrane. Results: The coaxial nanofibers exhibited a distinct core-shell structure and excellent antibacterial efficacy. H&E staining and VEGF immunohistochemistry confirmed that the coaxial drug-loaded composite fiber membrane promoted collagen synthesis, accelerated neovascularization, and facilitated wound healing. Conclusion: The prepared coaxial drug-loaded composite fiber membrane meets all the testing requirements and can effectively promote wound healing and inhibit bacteria, providing a new approach for the development of novel wound dressings.

关键词

姜黄素 / 静电纺丝技术 / 抑菌 / 复合纤维膜 / 伤口敷料

Key words

curcumin / electrospinning technology / bacteriostasis / composite fibrous membrane / wound dressing

[1] JI DX, LIN YG, GUO XY, et al. Electrospinning of nanofibres[J]. Nat Rev Meth Primers, 2024, 4: 1.
[2] TAN SM, TEOH XY, HWANG JL, et al. Electrospinning and its potential in fabricating pharmaceutical dosage form[J]. J Drug Deliv Sci Technol, 2022, 76: 103761.
[3] 师奇松, 于建香, 顾克壮, 等. 静电纺丝技术及其应用[J]. 化学世界, 2005, 2(5): 313-316.
[4] 屠卓隆, 林才. 姜黄素对糖尿病创面的促愈合作用及其机制研究进展[J]. 中华烧伤杂志, 2021, 37(4): 391-394.
[5] 李曼, 武丁胜, 魏安方, 等. 静电纺丝聚己内酯/明胶载姜黄素生物活性敷料的制备和性能[J]. 材料导报, 2022, 36(11): 233-239.
[6] 张梦星, 孙馨怡, 饶秉钧, 等. 海藻酸钠-壳聚糖-五倍子提取物微胶囊琼脂平皿扩散法抗菌性研究[J]. 纺织导报, 2016(11): 104-106.
[7] 张立颖, 李亚洁, 扬磊, 等. Wistar大鼠Ⅱ度烫伤模型的建立[J]. 护理研究, 2003, 17(11): 624-626.
[8] LIU N, XUE BX, CHENG W, et al. Healing mechanism of cotton bandages loaded with PNIPAM/GO-Ag hydrogel on deep second-degree burn wounds in a rat model[J]. Burns, 2024, 50(9): 107235.
[9] 单颖慧. 载药静电纺丝纳米纤维用于创伤愈合及疤痕抑制的研究[D]. 杭州: 浙江大学, 2015.
[10] 张倞, 康雨欣, 余华庆, 等. 不同烧伤深度大鼠模型建立的研究[J]. 山西医科大学学报, 2024, 55(12): 1527-1534.
[11] 单如梦. 重组人源Ⅲ型胶原蛋白脂质体凝胶剂的制备及其促创伤愈合的研究[D]. 济南: 山东大学, 2021.
[12] CHEN YX, ZHANG XR, LIU ZH, et al. Obstruction of the formation of granulation tissue leads to delayed wound healing after scald burn injury in mice[J]. Burns Trauma, 2021, 9: tkab004.
[13] GUO JT, WANG TY, YAN ZF, et al. Preparation and evaluation of dual drug-loaded nanofiber membranes based on coaxial electrostatic spinning technology[J]. Int J Pharm, 2022, 629: 122410.
[14] LIU XK, XU HX, ZHANG MX, et al. Electrospun medicated nanofibers for wound healing: review[J]. Membranes (Basel), 2021, 11(10): 770.
[15] 祖雨婷, 贾其松, 杨昱彤, 等. 抗菌促愈合伤口敷料的制备及研究进展[J]. 辽宁丝绸, 2024, 5(4): 94-95.

基金资助

2024年度辽宁省教育厅基本科研项目(理工类)(LJ212410140024)