乃孜来颗粒的抗炎与镇痛作用及机制研究

Study on the anti-inflammatory and analgesic effects of Naizilai Granules and their mechanisms

摘要

目的: 评价乃孜来颗粒的抗炎、镇痛作用,并初步探讨其作用机制。方法: 采用冰醋酸致小鼠扭体和蛋清致小鼠足趾肿胀实验观察乃孜来颗粒的抗炎、镇痛作用,并测定小鼠血清中肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-12(interleukin-12,IL-12)和白细胞介素-1β(interleukin-1β,IL-1β)的含量;观察乃孜来颗粒对脂多糖(lipopolysaccharide,LPS)致炎RAW264.7细胞的抑制作用及其上清液中炎症因子IL-12、IL-1β、TNF-α的表达,并通过蛋白免疫印迹法(Western blot,WB)检测环氧合酶-2(cyclooxygenase-2,COX-2)和前列腺素E₂(prostaglandin E₂,PGE₂)蛋白的表达。结果: 冰醋酸致小鼠扭体实验结果显示,与模型组相比,乃孜来颗粒高、中、低剂量组小鼠扭体次数均显著减少;蛋清致小鼠足趾肿胀实验结果显示,与模型组相比,乃孜来颗粒高、中、低剂量组小鼠足趾肿胀程度显著降低;乃孜来颗粒抑制LPS致炎后RAW264.7细胞的增殖,降低上清液中IL-12、IL-1β、TNF-α的含量。此外,乃孜来颗粒显著降低COX-2和PGE₂的含量。结论: 乃孜来颗粒具有抗炎、镇痛的药理作用。

Abstract

Objective: To evaluate the anti-inflammatory and analgesic effects of Naizilai Granules (NZL) and to preliminarily investigate the underlying mechanisms. Methods: The anti-inflammatory and analgesic effects of NZL were evaluated in mice using the acetic acid-induced writhing test as well as the egg white-induced paw edema model. Serum levels of tumor necrosis factor-α (TNF-α), interleukin-12 (IL-12), and interleukin-1 beta (IL-1β) were quantified by enzyme-linked immunosorbent assay (ELISA). In lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages, the inhibitory effect of NZL on inflammation was assessed. Additionally, the cytokine levels (IL-12, IL-1β and TNF-α) in cell supernatants were measured, and the protein expression of cyclooxygenase-2 (COX-2) and prostaglandin E₂ (PGE₂) was detected by Western blot (WB). Results: The acetic acid-induced writhing test demonstrated a significant reduction in the number of writhes in NZL-treat groups compared to the model group. In the egg white-induced paw edema model, NZL treatment groups showed markedly decreased paw swelling compared to the model group. Furthermore, NZL effectively inhibited the LPS-induced inflammation in RAW264.7 cells and decreased the expression levels of IL-12, IL-1β, and TNF-α in cell supernatants. Additionally, there was a marked reduction in the expression of COX-2 and PGE₂. Conclusions: NZL exhibits analgesic and anti-inflammatory effects.

关键词

乃孜来颗粒 / 抗炎 / 镇痛 / 巨噬细胞 / 脂多糖

Key words

Naizilai Granules / anti-inflammatory / analgesic / macrophages / lipopolysaccharide

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基金资助

2022年自治区科技重大专项:《基于成果转化的中药民族药临床应用研究》(2022A03008);新疆高发病的中药(民族药)创新药研发、大品种二次开发与产业化(2025A03007&2025A03007-1);药品监管科学全国重点实验室课题“维药乃孜来颗粒等一类创新药全方质控方法及标准研究”(2025SKLDRS0304)