大白菜蜡质缺失突变体YW71的遗传及序列变异分析

Inheritance and sequence variation analysis of a wax-less mutant YW71 in Chinese cabbage (Brassica rapa L. ssp. pekinensis)

摘要

以大白菜蜡质突变体YW71为对象，研究其亮绿无蜡粉性状的遗传规律和调控基因。通过遗传分析，表明YW71中的亮绿无蜡粉性状由隐性单基因控制。通过等位基因互补实验证明YW71的亮绿性状是 BrWAX2等位突变引起的。序列分析表明，在YW71中 BrWAX2基因在第3个外显子末端发生了39 bp的缺失，进而引起转录水平的可变剪切和翻译水平的提前终止。表达模式分析表明， BrWAX2基因在YW71茎和叶中表达量显著下降。此外，研究针对39 bp的变异开发并验证了共显性标记BrWAX2-InDel1。研究结果丰富了白菜类蔬菜蜡质突变遗传资源，将为亮绿无蜡粉品种的分子育种提供理论指导和技术支持。

Abstract

In this study, we identified a wax-less Chinese cabbage (Brassica rapa) mutant YW71, the inheritance and the controlling gene for the glossy trait were studied extensively. Genetic analysis indicated that the glossy trait in YW71 was controlled by a single recessive locus. Allelic complementary experiment showed that the glossy trait in YW71 was caused by mutation of the gene BrWAX2. Sequence analysis revealed that a 39 bp deletion was identified at the end of the third exon of BrWAX2, resulting in alternative splicing at the transcription level and finally leading to a premature stop codon at the translation level. Expression analysis showed that BrWAX2 was significantly down-regulated in stems and leaves of glossy YW71. Furthermore, a co-dominant marker BrWAX2-InDel was developed and validated. Overall, these results enrich the genetic resources of glossy mutants and provide applicable markers for marker-assisted selection (MAS)-based breeding of Chinese cabbage, which has pivotal significance in theory and practice.

关键词

大白菜 / 亮绿突变体 / 等位基因互补实验 / 序列变异分析

Key words

Chinese cabbage / Glossy mutant / Allelic complementary experiment / Sequence variation analysis

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基金资助

河南省农业科学院优秀青年科技基金(2022YQ10)

河南省自然科学基金(222300420197)

国家自然科学基金(32202485)

河南省农业科学院科技创新团队(2022TD06)

河南省重点研发专项(221111110100)

河南省农业良种联合攻关项目(2022010504)

农业农村部现代农业产业技术体系建设专项资金(CARS-23-G15)