淹水胁迫对菊芋幼苗生长形态及生理特性的影响

Effects of waterlogging stress on growth morphology and physiological characteristics of jerusalem artichoke seedling

摘要

为探究菊芋耐涝机制，以不淹水为对照，设 0、3、9 d 等 3 个淹水时间，共 6 个处理，研究了淹水胁迫对本地白皮菊芋的生长形态和生理特性的影响。结果表明，淹水 3、9 d, 菊芋的叶绿素含量、地上部干质量和地下部鲜干质量都显著低于对照；淹水 3 d 时，菊芋的可溶性糖含量、脯氨酸(Pro)含量和乳酸脱氢酶(LDH)活性均显著高于对照，分别比对照增加了 114.15%、449.02%、333.33%；淹水 9 d 时，菊芋的茎粗和过氧化物酶(POD)活性均显著高于对照，分别比对照增加了 11.23%、100.00%。说明本地白皮菊芋比较耐淹，通过提高其 LDH 活性进行乳酸发酵、提高 POD 活性、增加可溶性糖和 Pro 含量来适应淹水胁迫，使得植株少受或免受伤害。

Abstract

In order to explore the waterlogging tolerance mechanism of jerusalem artichoke (Helianthus tuberosus L.), a two-factor completely random design was adopted, the first factor was the waterlogging mode, and two levels of waterlogging and non-waterlogging (control) were set, and the second factor was the waterlogging time, and three levels of waterlogging were set to 0, 3 and 9 days, a total of 6 treatments were used to study the effects of waterlogging stress on the growth morphology and physiological characteristics of the local white bark jerusalem artichoke. The results showed that aboveground dry mass, underground fresh and dry mass of jerusalem artichoke seedlings were significantly lower than that of the control, as well as the chlorophyll content. The soluble sugarcontent, proline content and lactate dehydrogenase activity (LDH) of jerusalem artichoke seedlings were significantly higher than the control after 3 days of waterlogging stress, increased by 114.15%, 449.02% and 333.33%, respectively. Stem diameter and peroxidase (POD) activity were significantly higher than the control after 9 days of waterlogging stress, increased by 11.28% and 100.00%, respectively. In summary, native white bark jerusalem artichoke was a waterlogging tolerant plant. It adapted to waterlogging stress by increasing LDH activity, POD activity, soluble sugar and Pro content, so that the plant was less harmed or protected from damage.

关键词

菊芋 / 淹水胁迫 / 生长形态 / 生理特性

Key words

Helianthus tuberosus L. / Waterlogging stress / Growth morphology / Physiological characteristic

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基金资助

湖北省第四批现代农业产业技术体系高品质蔬菜产业技术体系(2023HBSTX4-06)

湖北省重点研发计划项目(2022BBA0061)

湖北省自然科学基金项目(2023AFB1001)