魔芋软腐病病株与健株根系分泌物比较分析

Comparative analysis of root exudates between healthy and soft rot diseased Amorphophallus konjac

摘要

软腐病是魔芋栽培中危害最严重的病害之一，目前无有效防控药剂。为了解软腐病病害发生与魔芋根系分泌物变化之间的关系，利用超高效液相色谱串联傅里叶变换质谱(UHPLC-Q Exactive HF-X)分析魔芋健康植株(HK)与软腐病病株(DK)根系分泌物的差异。结果表明，健康与发病植株根系分泌物显著不同，鉴定到的差异代谢物有340种。HK中特有的代谢物有9种，其中苯丙烷和聚酮化合物有助于提高植物自身抗病性；DK中特有的代谢物有7种，其中麦芽六糖、乳糖-N-岩藻糖III有助于软腐病病菌识别、侵染魔芋根系。差异代谢通路聚类分析结果表明，与代谢途径相关的代谢物有212种，其中参与柠檬酸循环的柠檬酸含量在HK中上调，高于DK。研究结果为利用根系次生代谢物进行软腐病防控研究提供理论依据。

Abstract

Soft rot is one of the most serious diseases in konjac cultivation, and there is currently no effective chemical agents. In order to understand the relationship between the changes of konjac root exudates induced by soft rot disease, the differences of root exudates between healthy (HK) and soft rot diseased konjac (DK) were analyzed by high-performance liquid chromatography tandem mass Fourier transform (UHPLC-q Exactive HF-X). The results showed that the root exudates of healthy and diseased konjac were significantly different, and 340 different metabolites were identified. There were 9 special metabolites in HK, among which phenylpropane and polyketone compounds were helpful to improve the disease resistance of plants. There were 7 special metabolites in DK, among which maltohexaose and lacto- N-fucopentaose III contributed to the recognition and infection of soft rot bacteria in konjac roots. Cluster analysis of differential metabolic pathways revealed that there were 212 metabolites related to metabolic pathways, among which the tricarboxylic acid cycle of citric acid was up-regulated in HK, higher than in DK. The research results provide a theoretical basis for the use of secondary metabolites in root for soft rot prevention and control.

关键词

魔芋 / 根系分泌物 / 软腐病

Key words

Konjac / Root exudates / Soft rot diseased

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基金资助

国家自然科学基金面上项目(32072558)

湖北省种业高质量发展专项

湖北省农业科技创新中心创新团队项目(2021-620-000-001-01)