蜜瓜籽不同溶剂提取物活性成分及抗氧化能力分析

Active ingredients and antioxidant capacity analysis of different solvent extracts from melon seeds

摘要

以河套地区5个不同品种蜜瓜籽(华莱士、银蜜、西州密25号、玉妃和红蜜)为研究对象，分别采用蒸馏水、70%乙醇、70%丙酮等不同溶剂进行提取，测定其总黄酮、总多酚和总多糖含量并与抗氧化活性进行相关性分析。结果表明，不同溶剂提取物抗氧化效果不同，70%乙醇提取物清除DPPH自由基、ABTS⁺自由基和抗氧化还原能力最强；华莱士瓜籽70%乙醇提取物中总黄酮和总多酚含量(w,后同)最高，分别为94.74、35.90 mg·g^-1；总多糖含量最高的是华莱士瓜籽水提取物，达159.29 mg·g^-1。相关性分析表明，总多酚含量与DPPH自由基清除能力、ABTS⁺自由基能力和还原能力呈显著或极显著正相关，总黄酮含量与DPPH自由基清除能力、还原能力呈显著或极显著正相关。建议将70%乙醇作为河套蜜瓜籽的提取溶剂，为河套蜜瓜副产物的进一步开发、提取和利用提供理论依据。

Abstract

In this study, distilled water, 70% ethanol and 70% acetone respectively were used as solvent to extract flavonoids, polyphenols and polysaccharides from five different cultivars of melon seeds (Hualaishi, Yinmi, Xizhoumi, Yufei and Hongmi). The correlation analysis between bioactive components and their antioxidant ability were explored. The results showed that fractions extracted by 70% ethanol from all samples expressed the best anti-redox capacity by significantly scavenging DPPH and ABTS⁺ radicals. Moreover, fractions extracted by 70% ethanol from Hualaishi melon seeds had the highest amount of total flavonoids (94.74 mg·g^-1) and total polyphenols (35.90 mg·g^-1) and fractions extracted by distilled water from Hualaishi melon seeds had the highest amount of total polysaccharides (159.29 mg·g^-1). The results of correlation analysis showed that the amount of total flavonoids and total polyphenols had significant positive correlations with the ability of scavenging DPPH, ABTS⁺ radicals and anti-redox. In conclusion, 70% ethanol was a potential preferences for extracting bioactive components from melon seeds and the findings would establish a theoretical basis for further research of by-products produced by Hetao melon.

关键词

蜜瓜籽 / 抗氧化活性 / 总黄酮 / 总多酚 / 总多糖

Key words

Melon seed / Antioxidant activity / Total flavonoids / Total polyphenols / Total polysaccharides

参考文献

[1] 徐莉莉, 刘海英, 刘静, 等. 成熟度及1-MCP对河套蜜瓜软化中物质代谢的影响[J]. 食品工业, 2017, 38(10): 150-154.

[2] 刘聪, 李亚珍, 尹嘉敏, 等. 不同贮藏温度对磴口华莱士蜜瓜质构特性、理化指标、感官品质变化及相关性分析[J]. 中国瓜菜, 2022, 35(1): 47-53.

[3] DI M S, VENDITTI P. Evolution of the knowledge of free radicals and other oxidants[J]. Oxidative Medicine and Cellular Longevity, 2020: 9829176.

[4] 李兴太, 纪莹. 线粒体氧化应激与天然抗氧化剂研究进展[J]. 食品科学, 2015, 36(7): 268-277.

[5] TADESSE D, RETTA N, GIRMA M, et al. In vitro antioxidant activities of plant polyphenol extracts and their combined effect with flaxseed on raw and cooked breast muscle fatty acid content, lipid health indices and oxidative stability in slow-growing sasso chickens[J]. Foods, 2023, 12(1): 115.

[6] GUO X L, REN T Z, LI H B, et al. Antioxidant activity and mechanism exploration for microwave-assisted extraction of flavonoids from Scutellariae radix using natural deep eutectic solvent[J]. Microchemical Journal, 2024, 200: 110300.

[7] LI H, ZHAI B T, SUN J, et al. Antioxidant, anti-aging and organ protective effects of total saponins from Aralia taibaiensis[J]. Drug Design Development and Therapy, 2021, 15: 4025-4042.

[8] 桂意云, 贤武, 梁强, 等. 甘蔗叶片多糖的提取及体外抗氧化作用[J]. 西南农业学报, 2012, 25(4): 1218-1221.

[9] 欧小球, 唐瀚, 谢向阳, 等. 我国西瓜甜瓜种业发展存在的问题及对策[J]. 中国瓜菜, 2014, 27(4): 75-76.

[10] 徐颖, 樊明涛, 冉军舰, 等. 不同品种苹果籽总酚含量与抗氧化相关性研究[J]. 食品科学, 2015, 36(1): 79-83.

[11] 何玉柱, 王琼琼, 崔瑞国, 等. 不同提取方法对红花籽油品质的影响及其抗氧化性的研究[J]. 食品工业科技, 2024, 45(20): 262-270.

[12] 慕钰文, 黄玉龙, 张辉元. 花椒籽不同溶剂提取物的活性成分及抗氧化性比较[J]. 包装与食品机械, 2021, 39(3): 21-26.

[13] 牛培荣, 李炜, 夏美如, 等. 河套小麦胚芽源多肽的抗氧化活性研究[J]. 粮油食品科技, 2023, 31(4): 87-94.

[14] 张福娟, 孙成行, 李思凡, 等. 绿豆皮在不同溶剂中提取物的抗氧化成分及抗氧化活性研究[J]. 粮食与油脂, 2021, 34(4): 151-155.

[15] 郭刚军, 胡小静, 付镓榕, 等. 澳洲坚果青皮不同极性溶剂分步提取物功能成分与抗氧化活性及其相关性分析[J]. 食品科学, 2021, 42(7): 74-82.

[16] 张福娟, 苏靖昕, 王炜琪, 等. 花红果渣不同溶剂提取物的抗氧化成分及性能分析[J]. 粮食与油脂, 2021, 34(6): 141-145.

[17] 许瑞如, 张秀玲, 李振, 等. 桔梗根提取物不同溶剂萃取物的抗氧化活性[J]. 食品研究与开发, 2021, 42(1): 31-36.

[18] 张华, 郑建东, 马田林, 等. 响应面法优化微波提取夏枯草多糖及其抗氧化活性[J]. 化学研究与应用, 2020, 32(2): 264-272.

[19] 刘佳, 向卓亚, 朱柏雨, 等. 赶黄草叶不同溶剂提取物中活性成分、抗氧化活性及胰脂肪酶抑制活性对比[J]. 食品工业科技, 2024, 45(20): 223-231.

[20] 刘庆, 陈琼, 李化强, 等. 不同溶剂对石榴籽提取物抗氧化活性的影响[J]. 邵阳学院学报(自然科学版), 2020, 17(4): 70-76.

[21] 赵玉红, 师帅帅, 张立钢. ‘鲁赫’刺蔷薇叶不同溶剂提取物的活性成分及抗氧化性比较[J]. 现代食品科技, 2019, 35(5): 159-166.

[22] 穆兴燕, 吴胜, 郭银萍, 等. 不同溶剂提取对刺梨物质含量变化及抗氧化活性的影响[J]. 食品研究与开发, 2023, 44(17): 68-75.

[23] 朱雨馨, 王文斌, 王佳婧, 等. 富锗木耳菌丝体不同溶剂提取物的体外抗氧化能力[J]. 食品工业科技, 2024, 45(8): 21-28.

[24] 徐韶棠, 杨轶浠, 王艺瑾, 等. 4种植物提取物的体外抗氧化活性比较[J]. 食品研究与开发, 2022, 43(24): 66-72.

[25] LOPES A P, PETENUCI M E, GALUCH M B, et al. Evaluation of effect of different solvent mixtures on the phenolic compound extraction and antioxidant capacity of bitter melon (Momordica charantia) [J]. Chemical Papers, 2018, 72(11): 2945-2953.

[26] 宋亚静, 林前锦, 吴妍欣, 等. 茭白提取物及不同溶剂萃取物的抗氧化活性研究[J]. 粮食与油脂, 2023, 36(11): 138-143.

基金资助

内蒙古自治区高等学校科学研究项目(NJZY23048)

巴彦淖尔市科技创新驱动专项项目(K202115)

现代农业产业技术体系建设专项(CARS-25)