番茄异胡豆苷合成酶基因的全基因组鉴定和表达分析

Genome-wide identification and expression analysis of the strictosidine synthetase genes in tomato

摘要

异胡豆苷合成酶(strictosidine synthetase, STR)作为单萜吲哚类生物碱合成过程的关键酶，对吲哚类生物碱的合成起决定性作用。目前茶树、拟南芥等植物中已有相关研究证明 STR基因在逆境胁迫中发挥重要功能，但在番茄中仍未有研究。为研究番茄中 STR基因的功能，笔者从番茄基因组中鉴定出14个异胡豆苷合成酶基因(SlSTR1~14)，并对其理化性质、基因结构、系统进化及表达模式进行分析。理化分析结果表明，除SlSTR8、9、11、12和14为疏水性蛋白外，其余SlSTR均为亲水性蛋白，亚细胞定位预测显示所有SlSTR均位于液泡中。亲缘关系分析结果表明，番茄、拟南芥、水稻和茶树中的 STR家族基因不均匀地分布在6个类群中。顺式作用元件分析表明， SlSTR启动子区含有胁迫和激素响应有关的多种调控元件。基因表达分析结果表明， SlSTR基因主要在番茄茎、叶和花组织中表达。对番茄苗分别进行干旱与高温处理，结果表明，相较于处理前大部分基因在处理后表达量均显著升高，少数基因如 SlSTR1、 2、 8在干旱处理后表达量显著降低， SlSTR8、 11和 12在高温处理后表达量显著降低，这表明 STR基因家族在面对不同逆境胁迫时，其表达模式是不同的。

Abstract

As a key enzyme in monoterpene indole alkaloids biosynthesis, strictosidine synthetase (STR) plays a decisive role in the synthesis of indole alkaloids. At present, there are some reports about the STR genes function in Camellia sinensis, Arabidopsis thaliana and other plants under adversity stress. But the related studies have not been reported in tomato. In order to study the function of STR genes in tomato, 14 strictosidine synthase genes (SlSTR1-14) were identified from tomato genome, and their physicochemical properties, gene structure, phylogenetic evolution and expression patterns were also analyzed. The result of physicochemical analysis showed that all SlSTR except SlSTR8, 9, 11, 12 and 14, were hydrophilic proteins, and subcellular localization prediction showed that all SlSTR were located in vacuoles. Phylogenetic analysis showed that STR famliy genes were unevenly distributed in VI groups of Solanum lycopersicum, Arabidopsis, Oryza sativa and Camellia sinensis. Cis-acting element analysis showed that the promoter regions of SlSTR genes contain several regulatory elements related to stress and hormone responses. The results of gene expression analysis showed that SlSTR genes were mainly expressed in the stem, leaf and flower tissues of tomato. Drought and high-temperature treatments were applied to tomato seedlings, and the results showed that, compared to before treatment, the expression levels of most genes significantly increased after treatment. However, a few genes such as SlSTR1, 2, and 8 showed significantly decreased expression levels after drought treatment, while SlSTR8, 11, and 12 showed significantly decreased expression levels after high-temperature treatment. This indicates that the expression patterns of the STR gene family are different when facing different adversity stresses.

关键词

番茄 / 异胡豆苷合成酶 / 鉴定 / 表达分析

Key words

Tomato / Strictosidine synthetase / Identification / Expression analysis

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基金资助

国家自然科学基金青年基金项目(31902024)

河南省科技攻关项目(212102110413)

河南农业大学博士启动基金项目(30500495)