黄瓜枯萎病抗性的遗传分析

Genetic analysis of resistance of cucumber to Fusarium wilt

摘要

为研究黄瓜枯萎病抗性的遗传规律，选择华南生态型黄瓜枯萎病高感材料 Han203 为母本与华南生态型黄瓜枯萎病高抗材料 BLF-2-1A 为父本，华南生态型黄瓜枯萎病高感材料 NS 为母本与其近等基因系黄瓜枯萎病高抗材料 NR 为父本，分别杂交获得 2 个四世代杂交组合，采用主基因与多基因混合模型分析了黄瓜枯萎病的遗传规律。结果显示，Han203× BLF-2-1A 在 2018 年秋季符合 E-1(MX2-ADI-AD)模型，在 2019 年春季符合 E-0(MX2- ADI- ADI)模型；NS × NR 在 2023 年春季符合 E- 1(MX2- ADI- AD)模型，在 2023 年秋季符合 E- 0(MX2-ADI-ADI)模型。在这 4 个模型中，主基因遗传效率分别为 87.98%、77.30%、73.96%和 72.33%，多基因遗传效率分别为 0、2.7%、0 和 0，环境方差占表型方差百分比分别为 12.22%、22.70%、26.40%和 27.67%。研究结果为黄瓜枯萎病抗性材料的选育及挖掘黄瓜枯萎病抗性相关基因提供了参考依据。

Abstract

In order to study the genetic law of cucumber resistance to Fusarium wilt, in this study, two four-generation hybrid combinations were obtained by Han203 cross with BLF-2-1A, NS cross with NR, respectively. The inheritance law of cucumber wilt was analyzed by using a mixture of principal genes and multi-gene model. The results showed that Han203×BLF-2-1A conformed to the E-1(MX2-ADI-AD) model in autumn 2018 and the E-0(MX2-ADI-ADI) model in spring 2019; NS×NR conformed to the E-1(MX2-ADI-AD) model in spring 2023 and the E-0(MX2- ADI-ADI) model in 2023 autumn. In these four models, the main gene genetic efficiency was 87.98%, 77.30%, 73.96% and 72.33%, the polygenic genetic efficiency was 0, 2.7%, 0 and 0, and the environmental variance as a percentage of phenotypic variance was 12.22%, 22.70%, 26.40% and 27.67%, respectively. This study provides a reference basis for the selection and breeding of cucumber wilt resistance materials and the mining of cucumber wilt resistance related genes.

关键词

黄瓜 / 枯萎病 / 主基因+多基因 / 遗传分析

Key words

Cucumber / Fusarium wilt / Major gene + polygene / Genetic analysis

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基金资助

国家自然科学基金联合基金项目(U22A20498)

湖南省自然科学基金青年基金项目(2023JJ40389)

湖南省农业科技创新资金项目(2023CX03)