不同栽培条件对榆黄蘑生理特性及总黄酮含量的影响

Effects of different cultivation conditions on physiological properties and total flavonoids content of Pleurotus citrinopileatus

摘要

为探讨不同栽培条件对榆黄蘑生理特性及总黄酮含量的影响，以榆黄蘑为材料，采用超声波辅助提取法对13种不同栽培条件下的榆黄蘑进行总黄酮提取，并用亚硝酸钠-硝酸铝显色法进行显色分析。结果表明，在510 nm处， R²=0.995 9，线性关系良好；在不同栽培条件下，榆黄蘑子实体均检测出黄酮类化合物；在不同光照处理中，自然光处理榆黄蘑总黄酮含量(w,后同)最高，为22.607 mg·g^-1，红光处理总黄酮含量最低，为14.667 mg·g^-1；在不同栽培料处理中，混合栽培料处理榆黄蘑总黄酮含量最高，为22.007 mg·g^-1，玉米芯处理含量最低，为17.663 mg·g^-1；在不同部位的总黄酮含量分析中，菌盖总黄酮含量最高，为25.154 mg·g^-1，菌座总黄酮含量最低，为13.918 mg·g^-1。不同栽培条件影响榆黄蘑的黄酮含量，且对其生理特性也有一定的影响，栽培条件直接或间接地影响体内黄酮的积累与转移。

Abstract

In order to study the effect of different cultivation conditions on the physiological characteristics and total flavonoid content of Pleurotus citrinopileatus, the total flavonoid was extracted from 13 kinds of P. citrinopileatus under different cultivation conditions by ultrasonic-assisted extraction method, and the color analysis was carried out by sodium nitrite and aluminum nitrate. The results showed that the linear relationship was good at 510 nm (R ²=0.995 9). Flavonoids were detected in the P. citrinopileatus under different cultivation conditions. In different light treatments, the total flavonoid content of natural light treatment was the highest (22.607 mg·g^-1), while the red light treatment was the lowest (14.667 mg·g^-1). Among different cultivation materials, the mixed cultivation material treatment had the highest total flavonoid content of 22.007 mg·g^-1, while the corn cobs treatment had the lowest of 17.663 mg·g^-1. In the analysis of total flavonoid content in different parts, the content of total flavonoid in the cap of P. citrinopileatus was the highest (25.154 mg·g^-1), while the content of total flavonoid in the fungal seat was the lowest (13.918 mg·g^-1). Different cultivation conditions had influence on the flavonoid content and physiological characteristics of P. citrinopileatus. Cultivation conditions directly or indirectly affected the accumulation and transfer of flavonoid in the body.

关键词

榆黄蘑 / 生理特性 / 黄酮 / 超声波

Key words

Pleurotus citrinopileatus / Physiological characteristics / Flavonoids / Ultrasound

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基金资助

广西自然科学基金(2022GXNSFBA035524)

崇左市科技计划项目(崇科20220618)

广西民族师范学院服务地方专项(2022FW072)