NaCl胁迫对黄心菜种子萌发及幼苗生长的影响

Effects of NaCl stress on the germination of Brassica campestris seeds and seedling growth

摘要

探究NaCl胁迫对黄心菜种子萌发及幼苗生长的影响，为筛选耐盐种质资源、培育优良品种提供依据。以淮南塌地乌3号为试验材料，在0(CK)、0.05、0.1、0.2、0.3、0.4 mol·L^-1 NaCl浓度下进行种子萌发及幼苗生长胁迫试验，分别测定其种子萌发和幼苗相关的表型与生理生化指标。结果表明，黄心菜种子的发芽势、发芽率、胚根长、胚芽长随着NaCl浓度的升高极显著降低，与黄心菜幼苗期相比，其发芽期对盐胁迫更为敏感；在幼苗期，随着NaCl胁迫浓度的增加，黄心菜的根鲜干质量、地上部质量、叶宽表现为低浓度促进生长、高浓度抑制生长。幼苗的叶长、株高、叶宽则显著低于对照且随着胁迫浓度的增加逐渐降低，SPAD值呈现先显著增加后降低的趋势，MDA含量、脯氨酸(Pro)含量均显著高于对照，POD活性显著高于对照且呈现显著升高而后逐渐降低的趋势。综上所述，0.05 mol·L^-1 NaCl显著抑制黄心菜种子的发芽率与发芽势的升高，但对黄心菜胚芽生长有显著促进作用。NaCl胁迫处理浓度大于0.2 mol·L^-1时显著抑制幼苗的地上部鲜质量、干质量、株高的增加，MDA、Pro含量和过氧化物酶活性呈显著升高再降低的趋势。结果可作为评价NaCl胁迫下黄心菜耐盐性强弱的指标。

Abstract

By analyzing the effects of NaCl stress on seed germination and seedling growth of Brassica campestris, provide a basis for screening salt tolerant germplasm resources and cultivating excellent varieties. Huainan Tadiwu No. 3 was used as the experimental material to conduct seed germination and seedling growth stress tests at concentrations of 0, 0.05, 0.1, 0.2, 0.3, 0.4 mol·L^-1, and the phenotypic, physiological and biochemical indicators related to seed germination and seedlings were measured. The results showed that with the increase of NaCl concentration, the germination potential, germination percentage, radicle and plumule length of Brassica campestris seeds decreased significantly. Compared with the seedling stage of Brassica campestris, its germination stage was more sensitive to salt stress; At the seedling stage, with the increase of NaCl stress concentration, the root fresh weight, root dry weight, above ground mass, and leaf width of Brassica campestris exhibited low concentration promoting growth and high concentration inhibiting growth. The leaf length, plant height, and leaf width of the seedlings were significantly lower than the control and gradually decreased with the increase of stress concentration. The relative chlorophyll content showed a trend of first significant increase and then decrease, and the MDA content and proline content were significantly higher than the control. The POD activity was significantly higher than the control and showed a significant increase and then gradually decreased. In conclusion, the concentration of 0.05 mol·L^-1 NaCl significantly inhibited the germination rate and potential of Brassica campestris, but had a significant promoting effect on the plumule length of Brassica campestris embryos. When the concentration of NaCl solution was higher than 0.2 mol·L^-1, above ground fresh mass, dry mass, and plant height of seedlings was significantly inhibited. MDA and Pro content, peroxidase activity showed a significant increase and then decrease trend, which can be used as an indicator to evaluate the salt tolerance of Brassica campestris under NaCl stress.

关键词

黄心菜 / 盐胁迫 / 种子萌发 / 生理生化指标

Key words

Brassica campestris / Salt stress / Seed germination / Physiological and biochemical indexes

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基金资助

河南省高等学校重点科研项目(16A210054)