水杨酸对盐胁迫下苦瓜种子萌发及幼苗生长的影响
Effects of salicylic acid on seed germination and seedling growth of bitter gourd under salt stress
为探究水杨酸(SA)对苦瓜盐害的缓解作用,以苦瓜品种良苦 1 号为试材,采用拌种和叶面喷施的方法,研究不同浓度(0.001、0.01、0.1、1.0 mmol·L-1) SA 处理对 150 mmol·L-1 NaCl 胁迫下苦瓜种子萌发和幼苗生长的影响。结果表明,0.01 mmol·L-1 SA 处理显著提高了盐胁迫下苦瓜种子的发芽率、发芽势、发芽指数、活力指数及幼苗的株高、叶面积、根长、鲜质量、干质量。0.1 mmol·L-1 SA 处理显著提高了苦瓜种子的发芽率、发芽势及幼苗的叶面积、鲜质量、干质量,对种子的发芽指数、活力指数及幼苗的株高、根长无显著影响。0.01 mmol·L-1 SA 处理显著提高了苦瓜幼苗抗氧化酶(SOD、POD、CAT)的活性及脯氨酸、可溶性糖的含量,显著降低了丙二醛的含量。0.1 mmol·L-1 SA 处理显著提高了苦瓜幼苗 POD 的活性及脯氨酸、可溶性糖的含量,对 SOD、CAT 的活性及丙二醛的含量无显著影响。综上,施用一定浓度外源 SA 对苦瓜盐害具有缓解作用,0.01 mmol·L-1 SA 处理是缓解苦瓜盐害的最佳方案。
To explore the effect of salicylic acid (SA) on the growth of bitter gourd under salt stress, Liangku No. 1 bitter gourd was used as the test material, and the effects of different concentrations (0.001, 0.01, 0.1, 1.0 mmol · L-1) SA on seed germination and seedling growth of bitter gourd under 150 mmol·L-1 NaCl stress were studied. The results showed that 0.01 mmol · L-1 SA treatment significantly improved the germination rate, germination potential, germination index, viability index, plant height, leaf area, root length, net mass, and dry mass of bitter gourd under salt stress. 0.1 mmol·L-1 SA treatment significantly improved the germination rate, germination potential, leaf area, net mass, and dry mass of bitter gourd, and had no significant effect on the germination index, vitality index, plant height, and root length of seedlings. 0.01 mmol · L-1 SA treatment significantly increased the activity of antioxidant enzymes (SOD, POD, CAT), the content of proline and soluble sugar, and significantly reduced the content of malondialdehyde in bitter gourd seedlings. 0.1 mmol·L-1 SA treatment significantly increased the POD activity and the content of proline and soluble sugar in bitter gourd seedlings but had no significant effect on the activity of SOD, CAT, and the content of malondialdehyde. In summary, applying exogenous SA at a certain concentration can alleviate the salt damage of bitter gourd. In this experiment, the best treatment concentration of SA was 0.01 mmol·L -1.
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聊城大学博士科研启动基金项目(318051823)
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