不同光质LED光源对星孢寄生菇生长发育的影响

Effects of LED light source with different light quality on the growth and development of Asterophora lycoperdoides

摘要

为探究星孢寄生菇在光胁迫下的生长发育特性，采用平板培养法，研究了10种不同光质LED光源对该菇菌丝生长、原基分化及子实体发育的影响。结果表明，与黑暗对照相比，不同光源对该菇菌丝生长均有抑制作用，其中白光的抑制效果最显著。同时，光源对该菇原基分化数量的影响不显著，但白光培养下的原基分化时间最长(3.75 d)。此外，暖黄光对该菇子实体发育有促进作用，蓝光有抑制作用，紫光有先抑制后促进的作用。接种后2~3 d, 该菇菌丝生长速度和原基分化数量呈线性增长趋势，在大多数光源下分别在第3、4天达到峰值；第3~4天，在大多数光源下，该菇子实体发育数量也呈线性增长趋势，并达到峰值。值得注意的是，在某些光源下，该菇菌丝生长速度、原基分化数量和子实体发育数量在高峰期后仍呈现小高峰。总之，光胁迫对该菇菌丝生长不利，对原基分化影响较小，但对子实体发育有正负两方面的影响。

Abstract

In order to investigate the growth and development characteristics of Asterophora lycoperdoides under light stress, the effects of 10 different light quality LED light sources on the mycelium growth, primordium differentiation and fruiting body development of this mushroom were investigated by plate culture method. The results showed that different light sources inhibited the mycelial growth of A. lycoperdoides, with white light showing the most significant inhibitory effect. Meanwhile, the effect of light source on the number of its primordium differentiation was not significant, but the longest time of primordium differentiation (3.75 d) was observed under white light culture. In addition, warm yellow light promoted the development of its fruiting body development, while blue light had an inhibitory effect, and violet light tended to inhibit and then promote the development of this mushroom. From 2 to 3 d after inoculation, the mycelium growth rate and the number of primordium differentiation of A. lycoperdoides showed a linear increasing trend, and reached the peaks on the 3 d, 4 d under most of the light sources, respectively; from 3 to 4 d, under most light sources, the number of its fruiting body development also showed a linear growth trend and reached a peak. It is worth noting that the mycelium growth rate, the number of primordium differentiation and the number of fruiting body development of this mushroom still showed small peaks after the peak period under some light sources. In conclusion, light stress was detrimental to the mycelium growth of A. lycoperdoides and had a minor effect on the primordium differentiation, but had a positive and negative effect on the development of the fruiting body.

关键词

星孢寄生菇 / LED光源 / 菌丝生长 / 原基分化 / 子实体发育

Key words

Asterophora lycoperdoides / LED light source / Mycelial growth / Primordial differentiation / Fruiting body development

参考文献

[1] 王建东, 赵建, 张杰, 等. 星孢寄生菇出菇培养及生物活性初探[J]. 四川大学学报(自然科学版), 2004, 41(1): 198-202.

[2] 卯晓岚. 中国大型真菌[M]. 郑州: 河南科学技术出版社, 2000.

[3] 庄严, 赵俊, 泰国夫, 等. 奥氏蜜环菌杂交双倍体子实体的人工诱导[J]. 中国森林病虫, 2003, 22(5): 13-16.

[4] 姜宝财. 食用菌栽培技术研究进展[J]. 中国农业信息, 2015(9): 91-92.

[5] 张浩, 张焕仕, 王猛, 等. 我国食用菌栽培技术研究进展[J]. 北方园艺, 2014(5): 175-179.

[6] 张金霞, 陈强, 黄晨阳, 等. 食用菌产业发展历史、现状与趋势[J]. 菌物学报, 2015, 34(4): 524-540.

[7] 张焱珍, 周会明, 白玉英, 等. 一株临沧野生灵芝的鉴定与驯化栽培[J]. 热带作物学报, 2020, 41(11): 2176-2182.

[8] 郑媛, 李仰华, 韩鹏杰, 等. 外源性物质对猪苓菌丝体生长及多糖含量的影响[J]. 中国实验方剂学杂志, 2021, 27(14): 129-137.

[9] 周会明. 食用菌栽培技术[M]. 北京: 中国农业大学出版社, 2017.

[10] 孙雅洁. 不同光质、光强对杏鲍菇生长发育的影响[D]. 河北保定: 河北农业大学, 2014.

[11] 魏生龙, 王治江, 于海萍, 等. 荷叶离褶伞分离驯化研究[J]. 西北农业学报, 2005, 14(5): 128-131.

[12] 周会明, 张焱珍, 柴红梅, 等. 灵芝原种及栽培种培养基配方筛选初探[J]. 食用菌, 2018, 40(2): 42-44.

[13] 唐拥政, 张忠, 周帅, 等. SPSS统计软件在食用菌研究中的应用[J]. 食用菌学报, 2014, 21(4): 81-86.

[14] 洪沛, 舒黎黎, 李天来, 等. 光环境对食用菌生长发育的影响[J]. 食用菌学报, 2021, 28(4): 108-115.

[15] 梅锡玲. 光质对灵芝生长、内源植物激素及三萜酸影响研究[D]. 北京: 北京协和医学院, 2014.

[16] MURAO S, SATOI S. Studies on persion inhibitor (S2PI) from Streptomyces naniniwaensis[J]. Agricultural and Biological Chemistry, 1971, 35: 1477-1484.

[17] ODANI S, TOMINAGA K, KONDOU S, et al. The inhibitory properties and primary structure of a novel serine proteinase inhibitor from the fruiting body of the basidiomycete, Lentinula edodes[J]. European Journal of Biochemistry, 1999, 262(3): 915-923.

[18] 郭征. 不同LED光源对秀珍菇生长发育的影响[D]. 合肥: 安徽农业大学, 2018.

[19] 于丽丽, 杨方, 王润涛, 等. LED光源对平菇菌丝生长影响的研究[J]. 农机化研究, 2015, 37(4): 155-158.

[20] BAEK I S, LEE Y H, JANG M J, et al. Effects of cultural characteristics of Lentinula edodes according to LED wavelength with sawdust substrate cultivation[J]. Journal of Mushroom, 2013, 11(4): 226-229.

[21] 冯伟林, 蔡为明, 金群力, 等. 不同光质的LED光源对工厂化金针菇菌株生长的影响[J]. 食药用菌, 2014, 22(5): 268-269.

[22] 常明昌. 食用菌栽培[M]. 北京: 中国农业出版社, 2002.

[23] 边银丙. 食用菌栽培学[M].3版. 北京: 高等教育出版社, 2017.

[24] 孙雅洁, 李明, 李守勉, 等. 不同光质对杏鲍菇生长发育的影响[J]. 北方园艺, 2014(19): 142-144.

[25] 程国侦, 徐永, 张曦文. 光质对海鲜菇子实体外观形态及产量的影响[J]. 中国食用菌, 2020, 39(10): 27-36.

[26] 佟希丹. 不同光质LED对黄白品种金针菇商品性状与产量的影响[D]. 长春: 吉林农业大学, 2012.

[27] 刘文科, 杨其长, 赵姣姣. LED光质对蟹味菇子实体生长的影响[J]. 中国食用菌, 2014, 33(1): 42-43.

[28] 黄兵. 不同光质LED对白灵菇商品性状及产量的影响[D]. 长春: 吉林农业大学, 2016.

基金资助

云南省地方本科高校(部分)基础研究联合专项面上项目(2019FH001(-059))