番木瓜环斑病毒甜瓜分离物的基因组及其侵染性克隆

The genome and infectious clone of papaya ringspot virus melon isolate

摘要

番木瓜环斑病毒(papaya ringspot virus, PRSV)是瓜类作物主要病毒之一，分析了甜瓜分离物 HaNHK10 的基因组序列和分子变异，构建了具有侵染性的全长 cDNA 克隆。结果显示，HaNHK10 分离物基因组全长为 10 332 nt, 与其他分离物的核苷酸和氨基酸序列一致性分别为 74.60%~97.80%和 85.30%~98.50%。基于全基因组序列的系统进化分析显示，HaNHK10 与来自中国的所有分离物均聚集于 II 组中，并与中国山东的西葫芦分离物 PRSV-SD 亲缘关系最近。接种试验显示，HaNHK10 分离物的全长 cDNA 克隆具有侵染性，它能系统侵染甜瓜、黄瓜、西瓜、南瓜、西葫芦和瓠瓜 6 种作物，经接种产生的病毒后代也能够通过摩擦接种侵染植株。

Abstract

Papaya ringspot virus (PRSV) is one of the most important viruses in cucurbits crops. In this study, the genome sequence of melon isolate HaNHK10 was cloned and analyzed, and its full-length cDNA infectious clone was constructed. The results showed that the genome of isolate HaNHK10 was 10 332 nt in length, and the identities of the genome nucleotide sequence and amino acid sequence between HaNHK10 and other isolates were 74.60%-97.80% and 85.30%-98.50%, respectively. Phylogenetic analysis based on genome sequences showed that HaNHK10 and all the isolates from China were clustered in group Ⅱ, and zucchini isolate PRSV-SD from Shandong province was most closely related to HaNHK10. The inoculation showed that the infectious clone was successfully constructed, and it could systematically infect melon, cucumber, watermelon, pumpkin, zucchini and bottle gourd. The progeny produced from the clone was infectious by mechanical inoculation.

关键词

番木瓜环斑病毒 / 甜瓜分离物 / 基因组 / 侵染性克隆

Key words

Papaya ringspot virus / Melon isolate / Genome / Infectious clone

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基金资助

河南省科技攻关项目(202102110075)

国家现代农业产业技术体系(CARS-25-2023-G20)

中国农业科学院科技创新工程(CAAS-ASTIP-2022-ZFRI-09)