马铃薯 StWRKY51基因的克隆及在高温胁迫下的表达分析

Cloning and expression analysis of StWRKY51 gene in potato under heat stress

摘要

为研究马铃薯 StWRKY51基因在高温胁迫下的响应特性，利用 RT-PCR 技术从 Russet Burbank 马铃薯叶片中克隆得到 StWRKY51基因，并对其进行了生物信息学分析和高温胁迫下的表达分析。结果表明，马铃薯 StWRKY51基因开放阅读框为 696 bp, 编码 231 个氨基酸，与潘那利番茄、番茄和辣椒的氨基酸序列相似性高于 80%。生物信息学分析表明，StWRKY51 属于酸性稳定亲水性蛋白，其二级结构元件中无规则卷曲比例最高达 63.64%。结构域分析显示，StWRKY51 含有 1 个典型的 WRKY 保守结构域，锌指结构为 C2H2 型，属于第 II 亚家族。荧光定量 PCR 结果显示，马铃薯 StWRKY51基因在高温处理后被显著诱导表达，表明 StWRKY51基因可能在马铃薯抵御高温胁迫中起到一定的调控作用。该结果为进一步探究 StWRKY51在马铃薯热胁迫中发挥的功能提供参考。

Abstract

To explore the response characteristics of potato StWRKY51 gene under high temperature stress, the StWRKY51 gene was cloned from leaf of the potato cultivar Russet Burbank by RT-PCR technology, and bioinformatics analysis of this gene and the real-time fluorescence quantitative PCR for StWRKY51 expression in potato under the high temperature stress were performed in this study. The results were as follows: The open reading frame of StWRKY51 gene is 696 bp, encoded 231 amino acids; the amino acid sequence of StWRKY51 protein was more than 80% similar to that of Solanum pennellii, Solanum lycopersicum and Capsicum annuum; the StWRKY51 was belong to an acidic and stable hydrophilic protein, and the ratio of irregular curl in the secondary structure of protein was up to 63.64%. The domain analysis showed that StWRKY51 gene encoding protein had a typical WRKY conserved domain, and the zinc finger structure is C2H2 type, belonging to the Group Ⅱsubfamily. The qPCR results showed that the expression of StWRKY51 gene was significantly induced under high temperature treatment, indicating that StWRKY51 gene may play a regulation role in potato resistance to high temperature stress. The research provided a reference for further study on the function of StWRKY51 response to high temperature stress in potato.

关键词

马铃薯 / WRKY转录因子 / 基因克隆 / 表达分析

Key words

Potato / WRKY transcription factor / Gene cloning / Expression analysis

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基金资助

河南省科技攻关计划项目(242102110258)

河南省高等学校重点科研项目(24A210027)

郑州师范学院高层次人才科研启动专项经费(2018)

郑州师范学院“提质工程”项目(ZHJX-2022001232253)

郑州师范学院开放研究基金资助项目(2019)