44份西瓜种质资源苗期耐盐性综合评价

Comprehensive evaluation of salt tolerance at seedling stage of 44 watermelon germplasm resources

摘要

以44份西瓜种质为试验材料，设置0(CK)和150 mmol·L^-1 NaCl 2个处理水平，测定不同西瓜种质幼苗的15项指标。以15项指标的耐盐系数作为耐盐性评价依据，运用相关性分析、主成分分析、隶属函数值法、聚类分析等多元分析方法进行综合评价。通过主成分分析将15个单项指标转换为4个相互独立的综合指标，累计贡献率为71.10%；以耐盐系数为基础进行聚类分析，筛选出10个高度耐盐、21个中度耐盐、13个弱耐盐西瓜种质资源。采用隶属函数法进行综合评价，排名前5的种质分别是HY-91、DQ-308、W19-15、HY-73、W19-10，归为聚类分析中的第一类，属于强耐盐性品种。

Abstract

Fourty-four watermelon germplasms were used as experimental materials, and 0(CK) and 150 mmol·L^-1 NaCl was used at two treatment levels, and 15 indexes of different watermelon germplasm seedlings were determined. The salt tolerance coefficient of 15 indicators was used as the basis for salt tolerance evaluation, and multivariate analysis methods such as correlation analysis, principal component analysis, membership function value method and cluster analysis were used for comprehensive evaluation. The 15 individual indicators were converted into 4 independent comprehensive indicators by principal component analysis, and the cumulative contribution rate was 71.10%. The cluster analysis was carried out on the basis of the salt tolerance coefficient, and 10 highly salt-tolerant, 21 moderately salt-tolerant and 13 weakly salt-tolerant watermelon germplasm resources were screened out according to the size of the D value. The comprehensive salt tolerance evaluation value of each germplasm was obtained by the membership function value method, the top 5 were HY-91, DQ-308, W19-15, HY-73 and W19-10, belonging to highly salt-tolerant germplasm.

关键词

西瓜 / 苗期 / 耐盐性 / 隶属函数 / 聚类分析

Key words

Watermelon / Seedling stage / Salt tolerance / Membership function / Cluster analysis

参考文献

[1] 温利强. 我国盐渍土的成因及分布特征[D]. 合肥: 合肥工业大学, 2010.

[2] 杨真, 王宝山. 中国盐渍土资源现状及改良利用对策[J]. 山东农业科学, 2015, 47(4): 125-130.

[3] 周和平, 张立新, 禹锋, 等. 我国盐碱地改良技术综述及展望[J]. 现代农业科技, 2007(11): 159-161.

[4] VAN ZELM E, ZHANG Y X, TESTERINK C. Salt tolerance mechanisms of plants[J]. Annual Review of Plant Biology, 2020, 71(1): 403-433.

[5] ZORB C, GEILFUS C M, DIETZ K J. Salinity and crop yield[J]. Plant Biology, 2019, 21(S1): 31-38.

[6] 刘文革, 徐小利, 潘秀清, 等. 黄河故道地区西瓜甜瓜产业分析和建议[J]. 中国瓜菜, 2022, 35(8): 1-11.

[7] 束秀玉. CO₂加富对盐胁迫下西瓜幼苗生长及生理生化特性的影响[J]. 河南农业科学, 2020, 49(12): 107-114.

[8] 李彦, 张英鹏, 孙明, 等. 盐分胁迫对植物的影响及植物耐盐机理研究进展[J]. 中国农学通报, 2008, 24(1): 258-265.

[9] 高博文, 孙德玺, 刘君璞, 等. 盐胁迫对西瓜幼苗生理生化特性的影响[J]. 中国瓜菜, 2022, 35(8): 35-41.

[10] 杨发斌, 杨晓刚, 薛梦迪, 等. 18个大白菜品种耐盐性筛选[J]. 山东农业科学, 2017, 49(3): 31-36.

[11] 张涛, 刘勇鹏, 韩娅楠, 等. 120份辣椒种质资源苗期耐盐性研究[J]. 河南农业大学学报, 2020, 54(5): 793-802.

[12] 张蕾琛, 邢乃林, 应泉盛, 等. 不同类型南瓜耐盐材料的筛选[J]. 中国瓜菜, 2017, 30(6): 9-13.

[13] 李世玉, 程登虎, 闫星, 等. 26份甜瓜材料耐盐性鉴定与评价[J]. 中国瓜菜, 2022, 35(9): 23-30.

[14] 张云起. 设施西瓜耐盐砧木筛选及其耐盐机理研究[D]. 山东泰安: 山东农业大学, 2004.

[15] YAN Y Y, WANG S S, WEI M, et al. Effect of different rootstocks on the salt stress tolerance in watermelon seedlings[J]. Horticultural Plant Journal, 2018, 4(6): 239-249.

[16] 崔聪聪. 西瓜耐盐砧木的筛选及其在盐胁迫下的生理响应[D]. 南京: 南京农业大学, 2008.

[17] 阎志红, 刘文革, 赵胜杰, 等. NaCl胁迫对不同西瓜种质资源发芽的影响[J]. 植物遗传资源学报, 2006, 7(2): 220-225.

[18] 韩志平. 盐胁迫对小型西瓜生长、生理代谢的影响及外源钙和腐胺的缓解效应研究[D]. 南京: 南京农业大学, 2008.

[19] 高博文, 孙德玺, 袁高鹏, 等. 121份西瓜材料幼苗期耐盐性鉴定[J]. 果树学报, 2022, 39(9): 1597-1606.

[20] 张明亚, 庞胜群, 刘玉东, 等. 加工番茄种子萌发期耐盐性综合评价[J]. 中国瓜菜, 2023, 36(10): 84-90.

[21] 齐琪, 马书荣, 徐维东. 盐胁迫对植物生长的影响及耐盐生理机制研究进展[J]. 分子植物育种, 2020, 18(8): 2741-2746.

[22] KEREPESI I, GALIBA G. Osmotic and salt stress-induced alteration in soluble carbohydrate content in wheat seedlings[J]. Crop Science, 2000, 40(2): 482-487.

[23] MA Q L, KANG J M, LONG R C, et al. Proteomic analysis of salt and osmotic-drought stress in alfalfa seedlings[J]. Journal of Integrative Agriculture, 2016, 15(10): 2266-2278.

[24] 姜景彬, 魏民, 张贺, 等. 蔬菜耐盐性鉴定方法研究进展[J]. 中国瓜菜, 2009, 22(3): 39-41.

[25] ASHRAF M, HARRIS P. Potential biochemical indicators of salinity tolerance in plants[J]. Plant Science, 2004, 166(1): 3-16.

[26] HUANG Y, CAO H S, YANG L, et al. Tissue-specific respiratory burst oxidase homolog-dependent H₂O₂ signaling to the plasma membrane H⁺-ATPase confers potassium uptake and salinity tolerance in Cucurbitaceae[J]. Journal of Experimental Botany, 2019, 70(20): 5879-5893.

[27] 李晓芬, 尚庆茂, 张志刚, 等. 多元统计分析方法在辣椒品种耐盐性评价中的应用[J]. 园艺学报, 2008, 35(3): 351-356.

[28] 马帅国, 田蓉蓉, 胡慧, 等. 粳稻种质资源苗期耐盐性综合评价与筛选[J]. 植物遗传资源学报, 2020, 21(5): 1089-1101.

[29] 韩志平, 郭世荣, 李娟. 设施栽培西瓜品种对盐胁迫的响应[J]. 江苏农业学报, 2008, 24(6): 888-895.

基金资助

国家现代农业产业技术体系(CARS-25)

河南省科技攻关项目(232102110223)

河南省重大科技专项(221100110400)