南方根结线虫对7种瓜菜作物根系分泌物的趋性

Relationship between root exudates of cucurbits and vegetables plants and chemotaxis behavior of Meloidogyne incognita

摘要

为探究植物根系分泌物组分与南方根结线虫趋化性的关系，盆栽种植7种不同瓜菜作物(番茄、甜椒、苦瓜、蕹菜、芹菜、黄瓜、甜瓜)，收集并测定根系分泌物中初级代谢物和次生代谢物的含量，观察南方根结线虫J2s对不同瓜菜作物根系分泌物的行为趋性，并对根系分泌物各组分含量进行相关性分析。结果表明，南方根结线虫J2s对7种瓜菜作物的根系分泌物均表现出正趋向行为，其中J2s对蕹菜根系分泌物的趋化指数最高，为0.52；且趋化指数与根系分泌物中可溶性糖和蛋白质含量没有显著相关性，但与单宁、丁二酸、丙氨酸含量呈显著负相关，相关系数分别为-0.444、-0.527、-0.522，与生物碱、甘氨酸含量呈极显著负相关，相关系数分别为-0.601、-0.550。研究结果可为利用趋性技术防控南方根结线虫提供理论基础。

Abstract

To investigate the relationship between the components of root exudates and the chemotaxis of Meloidogyne incognita J2s, this study potted 7 different cucurbits and vegetables plants (tomato, sweet pepper, bitter gourd, water spinach, celery, cucumber, melon) and measured the primary metabolites and secondary metabolites extent of root exudates. Then, the tendency of M. incognita J2s towards different cucurbits and vegetables plants root exudates were observed. And the correlation analysis was also conducted between the chemotaxis of M. incognita J2s and the content of various components in plant root exudates. The results showed that M. incognita J2s exhibited a positive trend towards the root exudates of 7 cucurbits and vegetables plants, with J2s towards root exudates of Ipomoea aquatica having the highest chemotaxis index of 0.52. There was no significant correlation between chemotaxis and soluble sugars, proteins content, but significantly negatively correlated with tannin, succinic acid, alanine content in root exudates, with the correlation coefficients values of -0.444, -0.527, and -0.522, respectively, and extremely significantly negatively correlated with glycine and alkaloid content, with the correlation coefficients values of -0.601 and -0.550, respectively. The research results could provide a theoretical basis for using taxis technology to prevent and control M. incognita.

关键词

南方根结线虫 / 根系分泌物 / 趋化性 / 相关性

Key words

Meloidogyne incognita / Root exudates / Chemotaxis / Correlation

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基金资助

河北大学校长基金(XZJJ201924)

宁夏回族自治区重点研发计划(2021BBF02013)

河北省生物技术创新中心绩效补助经费项目(225676109H)