辣椒雄性不育的分子研究进展

张怡文 ,  徐兰婷 ,  王飞 ,  刘奕清 ,  姚明华 ,  徐凯

中国瓜菜 ›› 2024, Vol. 37 ›› Issue (2) : 1 -7.

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中国瓜菜 ›› 2024, Vol. 37 ›› Issue (2) : 1 -7. DOI: 10.16861/j.cnki.zggc.202423.0665
专题综述

辣椒雄性不育的分子研究进展

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Molecular research progress of male infertility in Capsicum annuum L.

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摘要

辣椒(Capsicum annuum L.)是世界上重要的蔬菜作物之一,杂种优势明显。利用雄性不育系制种可有效解决人工去雄的难题,简化制种工序,降低生产成本。而辣椒雄性不育是一个快速发展的研究领域。综述了近几年在辣椒雄性不育的类型与特点、细胞学特征、细胞核雄性不育的分子机制,以及细胞质雄性不育的机制解析等方面所取得的重要进展,并对辣椒雄性不育中存在的问题以及未来发展方向进行了讨论和展望,旨在为辣椒三系配套制种提供理论参考。

Abstract

Pepper (Capsicum annuum L.) is one of the important vegetable crops in the world, with obvious heterosis. Using the male sterile line to produce hybrids can effectively replace manual emasculation, thereby simplifying the seed production process and reducing production costs. The research on male infertility in pepper is a rapidly developing field. We reviewed the important progress in the types, characteristics and cytological features of male infertility, the molecular mechanism of genic male sterile and cytoplasm male sterility in pepper. Further, the problems and development directions of male infertility in pepper were discussed and prospected, aiming to provide a theoretical reference for the three-line system of hybrid seed production for pepper.

关键词

辣椒 / 细胞核雄性不育 / 细胞质雄性不育 / 细胞学特征

Key words

Capsicum annuum L. / Genic male sterile / Cytoplasm male sterility / Cytological features

引用本文

引用格式 ▾
张怡文,徐兰婷,王飞,刘奕清,姚明华,徐凯. 辣椒雄性不育的分子研究进展[J]. 中国瓜菜, 2024, 37(2): 1-7 DOI:10.16861/j.cnki.zggc.202423.0665

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参考文献

[1]

王立浩, 张宝玺, 张正海, 等. “十三五”我国辣椒育种研究进展、产业现状及展望[J]. 中国蔬菜, 2021(2): 21-29.

[2]

DANIELL H, CHASE C. Introduction to the molecular biology and biotechnology of plant organelles[M]// DANIELL H, CHASE C. Molecular biology and biotechnology of plant organelles: Chloroplasts and mitochondria. Dordrecht: Springer, 2004: 1-12.

[3]

ZHANG Z H, ZHU Y S, CAO Y C, et al. Fine mapping of the male fertility restoration gene CaRf032 in Capsicum annuum L.[J]. Theoretical and Applied Genetics, 2020, 133(4): 1177-1187.

[4]

DONG JC, HU F, GUAN W D, et al. A 163-bp insertion in the Capana10g000198 encoding a MYB transcription factor causes male sterility in pepper (Capsicum annuum L.)[J]. The Plant Journal, 2023, 113(3): 521-535.

[5]

CHEN Z, ZHAO N, LI S, et al. Plant mitochondrial genome evolution and cytoplasmic male sterility[J]. Critical Reviews in Plant Sciences, 2017, 36(1): 55-69.

[6]

CHASE C D. Cytoplasmic male sterility:A window to the world of plant mitochondrial-nuclear interactions[J]. Trends in Genetics, 2007, 23(2): 81-90.

[7]

程翔, 汤冰倩, 刘峰, 等. 辣椒雄性不育分子标记研究进展[J]. 辣椒杂志, 2020, 18(4): 40-45.

[8]

LIAO C C, YAN W, CHEN Z F, et al. Innovation and development of the third-generation hybrid rice technology[J]. Crop Journal, 2021, 9(3): 693-701.

[9]

LIN S W, SHIEH H C, WANG Y W, et al. Restorer breeding in sweet pepper:Introgressing Rf allele from hot pepper through marker-assisted backcrossing[J]. Scientia Horticulturae, 2016, 197: 170-175.

[10]

SWAMY B N, HEDAU N K, CHAUDHARI G V, et al. CMS system and its stimulation in hybrid seed production of Capsicum annuum L[J]. Scientia Horticulturae, 2017, 222: 175-179.

[11]

LUO D P, XU H, LIU Z L, et al. A detrimental mitochondrial-nuclear interaction causes cytoplasmic male sterility in rice[J]. Nature Genetics, 2013, 45(5): 573-577.

[12]

NIE Z X, CHEN J Y, SONG Y P, et al. Comparative transcriptome analysis of the anthers from the cytoplasmic male-sterile pepper line HZ1A and its maintainer line HZ1B[J]. Horticulturae, 2021, 7(12): 580.

[13]

GUO J J, WANG P, CHENG Q, et al. Proteomic analysis reveals strong mitochondrial involvement in cytoplasmic male sterility of pepper (Capsicum annuum L.)[J]. Journal of Proteomics, 2017, 168: 15-27.

[14]

CHENG Q, LI T, AI Y X, et al. Complementary transcriptomic and proteomic analysis reveals a complex network regulating pollen abortion in GMS (msc-1) pepper (Capsicum annuum L.)[J]. International Journal of Molecular Sciences, 2019, 20(7): 1789.

[15]

CHENG Q, LI T, AI Y X, et al. Phenotypic, genetic, and molecular function of msc-2, a genic male sterile mutant in pepper (Capsicum annuum L.)[J]. Theoretical and Applied Genetics, 2020, 133(3): 843-855.

[16]

JEONG K, CHOI D, LEE J. Fine mapping of the genic male-sterile ms1 gene in Capsicum annuum L[J]. Theoretical and Applied Genetics, 2018, 131(1): 183-191.

[17]

NARESH P, LIN S W, LIN C Y, et al. Molecular markers associated to two non-allelic genic male sterility genes in peppers (Capsicum annuum L.)[J]. Frontiers in Plant Science, 2018, 9: 1343.

[18]

张锐, 尚伟, 许旭明. 辣椒雄性不育的选育及利用研究进展[J]. 分子植物育种, 2020, 18(18): 6143-6157.

[19]

CHENG Q, WANG P, LIU J Q, et al. Identification of candidate genes underlying genic male-sterile msc-1 locus via genome resequencing in Capsicum annuum L[J]. Theoretical and Applied Genetics, 2018, 131(9): 1861-1872.

[20]

GUO J J, LIU C, WANG P, et al. The Aborted microspores (AMS)-like gene is required for anther and microspore development in pepper (Capsicum annuum L.)[J]. International Journal of Molecular Sciences, 2018, 19(5): 1341.

[21]

KIM Y J, ZHANG D B. Molecular control of male fertility for crop hybrid breeding[J]. Trends in Plant Science, 2018, 23(1): 53-65.

[22]

WANG K, GAO F, JI Y X, et al. ORFH79 impairs mitochondrial function via interaction with a subunit of electron transport chain complex III in Honglian cytoplasmic male sterile rice[J]. New Phytologist, 2013, 198(2): 408-418.

[23]

COLOMBO N, GALMARINI C R. The use of genetic, manual and chemical methods to control pollination in vegetable hybrid seed production:A review[J]. Plant Breeding, 2017, 136(3): 287-299.

[24]

PETERSON P A. Cytoplasmically inherited male sterility in Capsicum[J]. American Naturalist, 1958, 92(863): 111-119.

[25]

杨世周, 赵雪云. 辣椒 8021A 雄性不育系的选育及三系配套[J]. 中国蔬菜, 1984(3): 9-13.

[26]

JI J J, HUANG W, YIN C C, et al. Mitochondrial cytochrome c oxidase and F1Fo-ATPase dysfunction in peppers (Capsicum annuum L.) with cytoplasmic male sterility and its association with orf 507 and atp6-2 genes[J]. International Journal of Molecular Sciences, 2013, 14(1): 1050-1068.

[27]

KIM D H, KANG J G, KIM B D. Isolation and characterization of the cytoplasmic male sterility-associated orf456 gene of chili pepper (Capsicum annuum L.)[J]. Plant Molecular Biology, 2007, 63(4): 519-532.

[28]

JI J J, HUANG W, LI Z, et al. Tapetum-specific expression of a cytoplasmic orf507 gene causes semi-male sterility in transgenic peppers[J]. Frontiers in Plant Science, 2015, 6: 272.

[29]

LI J J, PANDEYA D, JO Y D, et al. Reduced activity of ATP synthase in mitochondria causes cytoplasmic male sterility in chili pepper[J]. Planta, 2013, 237(4): 1097-1109.

[30]

WEN J F, ZHAO K, LV J H, et al. Orf165 is associated with cytoplasmic male sterility in pepper[J]. Genetics and Molecular Biology, 2021, 44(3): e20210030.

[31]

WANG P, LU Q H, AI Y X, et al. Candidate gene selection for cytoplasmic male sterility in pepper (Capsicum annuum L.) through whole mitochondrial genome sequencing[J]. International Journal of Molecular Sciences, 2019, 20(3): 578.

[32]

郭金菊. 辣椒细胞质雄性不育花药的蛋白质组学分析及 CaSEP5 基因的功能分析[D]. 北京: 中国农业大学, 2018.

[33]

TANG H W, LUO D P, ZHOU D G, et al. The rice restorer Rf4 for wild-abortive cytoplasmic male sterility encodes a mitochondrial-localized PPR protein that functions in reduction of WA352 transcripts[J]. Molecular Plant, 2014, 7(9): 1497-1500.

[34]

HUANG W C, YU C C, HU J, et al. Pentatricopeptide-repeat family protein RF6 functions with hexokinase 6 to rescue rice cytoplasmic male sterility[J]. Proceedings of the National Academy of Sciences of the United States of America, 2015, 112(48): 14984-14989.

[35]

QIN X E, TIAN S K, ZHANG W L, et al. The main restorer Rf3 of maize S type cytoplasmic male sterility encodes a PPR protein that functions in reduction of the transcripts of orf355[J]. Molecular Plant, 2021, 14(12): 1961-1964.

[36]

NIE Z X, SONG Y P, WANG H, et al. Fine mapping and gene analysis of restorer-of-fertility gene CaRfHZ in pepper (Capsicum annuum L.)[J]. International Journal of Molecular Sciences, 2022, 23(14): 7633.

[37]

MANNA S. An overview of pentatricopeptide repeat proteins and their applications[J]. Biochimie, 2015, 113: 93-99.

[38]

GULYAS G, PAKOZDI K, LEE J S, et al. Analysis of fertility restoration by using cytoplasmic male-sterile red pepper (Capsicum annuum L.) lines[J]. Breeding Science, 2006, 56(3): 331-334.

[39]

WANG L H, ZHANG B X, LEFEBVRE V, et al. QTL analysis of fertility restoration in cytoplasmic male sterile pepper[J]. Theoretical and Applied Genetics, 2004, 109(5): 1058-1063.

[40]

KIM Y M, JO Y D, KWON J K, et al. Characterization and inheritance of a novel thermo-sensitive restoration of cytoplasmic male sterility in Capsicum annuum[J]. Scientia Horticulturae, 2013, 164: 512-520.

[41]

ZHANG B X, HUANG S W, YANG G M, et al. Two RAPD markers linked to a major fertility restorer gene in pepper[J]. Euphytica, 2000, 113(2): 155-161.

[42]

MIN W K, LIM H, LEE Y P, et al. Identification of a third haplotype of the sequence linked to the Restorer-of-fertility (Rf) gene and its implications for male-sterility phenotypes in peppers (Capsicum annuum L.)[J]. Molecules and Cells, 2008, 25(1): 20-29.

[43]

JO Y D, HA Y, LEE J H, et al. Fine mapping of Restorer-of-fertility in pepper (Capsicum annuum L.) identified a candidate gene encoding a pentatricopeptide repeat (PPR)-containing protein[J]. Theoretical and Applied Genetics, 2016, 129(10): 2003-2017.

[44]

ORTEGA F A, BARCHENGER D W, WEI B Q, et al. Development of a genotype-specific molecular marker associated with restoration-of-fertility (Rf) in chile pepper (Capsicum annuum)[J]. Euphytica, 2020, 216(3): 1-10.

[45]

KANG M C, KANG H J, JUNG S Y, et al. The unstable restorer-of-fertility locus in pepper (Capsicum annuum L.) is delimited to a genomic region containing PPR genes[J]. Theoretical and Applied Genetics, 2022, 135(6): 1923-1937.

[46]

CHENG J W, CHEN Y J, HU Y F, et al. Fine mapping of restorer-of-fertility gene based on high-density genetic mapping and collinearity analysis in pepper (Capsicum annuum L.)[J]. Theoretical and Applied Genetics, 2020, 133(3): 889-902.

[47]

WU L, WANG P, WANG Y H, et al. Genome-wide correlation of 36 agronomic traits in the 287 pepper (Capsicum) accessions obtained from the SLAF-seq-based GWAS[J]. International Journal of Molecular Sciences, 2019, 20(22): 5675.

[48]

于海龙, 任文静, 方智远, 等. 蔬菜细胞质雄性不育的育性恢复研究进展[J]. 园艺学报, 2021, 48(5): 1031-1046.

[49]

吕庆雪, 于彩虹, 李毅丹, 等. 浅析玉米杂交制种技术[J]. 分子植物育种, 2018, 16(12): 4037-4042.

[50]

魏兵强, 张淼, 王兰兰, 等. 辣椒胞质雄性不育及其育性恢复研究进展[J]. 生物技术通报, 2016, 32(4): 1-5.

[51]

LIU F, ZHAO J T, SUN H H, et al. Genomes of cultivated and wild Capsicum species provide insights into pepper domestication and population differentiation[J]. Nature Communications, 2023, 14(1): 5487.

[52]

LIAO Y, WANG J T, ZHU Z S, et al. The 3D architecture of the pepper genome and its relationship to function and evolution[J]. Nature Communications, 2022, 13(1): 3479.

基金资助

湖北省重点研发计划项目(2022BBA0061)

湖北省重点研发计划项目(2023BBB013)

湖北省重点研发计划项目(2023BBB044)

湖北省援疆援藏项目(2022BGD008)

湖北省农业科技创新中心项目(2021-620-000-001-007)

湖北省自然科学基金青年项目(2022CFC055)

湖北省支持种业高质量发展资金项目(HBZY2023B004-4)

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