干旱胁迫对丘北辣椒根际细菌群落结构的影响

Impact of drought stress on the rhizosphere bacterial community structure of Capsicum annuum in Qiubei

摘要

为探究干旱胁迫下丘北辣椒根系分泌物对根际微生物组的影响和塑造，通过盆栽控水方法，以正常种植为对照(CK)，干旱种植为处理(DS)，利用 Illumina-MiSeq 高通量测序技术分析不同处理下细菌群落的差异。结果表明，干旱胁迫导致辣椒叶绿素含量和根系活力显著降低，而可溶性糖、脯氨酸和丙二醛含量及超氧化物歧化酶活性显著提高。干旱处理显著降低细菌群落多样性指数(Chao1、Shannon 和 Simpson)，同时发现 CK 与干旱处理细菌群落结构相似度较小，具有显著差异。差异分析表明，干旱处理显著促进了放线菌门 Actinobacteria、中生根瘤菌属 Mesorhizobium 等有益微生物的富集。研究结果为丘北辣椒在干旱环境下适应机制研究及石漠化地区辣椒高效栽培提供了新的见解和思路。

Abstract

In order to investigate the effects of root exudates from Qiubei pepper under drought stress on rhizosphere microbial communities and their shaping, a pot experiment was conducted using controlled watering methods. Normal cultivation was used as the control (CK), while drought cultivation served as the treatment (DS). The differences in bacterial community were analyzed using Illumina-MiSeq high-throughput sequencing technology. The results revealed that drought stress significantly reduced chlorophyll content and root vitality of pepper. Conversely, soluble sugar, proline, malondialdehyde content, and superoxide dismutase activity exhibited significant increases. Drought treatment notably decreased bacterial community diversity indices (Chao1, Shannon, and Simpson). Furthermore, it was observed that CK and drought treatments displayed limited similarity in bacterial community structure with notable differences. Differential analysis demonstrated that drought treatment effectively promoted enrichment of beneficial microorganisms such as Actinobacteria and Mesorhizobium genus in rhizosphere soil. These findings provide novel insights into the adaptation mechanisms of Qiubei pepper under drought conditions and offer efficient cultivation strategies for peppers in rocky desertification areas.

关键词

丘北辣椒 / 干旱胁迫 / 16s rRNA / 根际细菌

Key words

Qiubei pepper / Drought stress / 16s rRNA / Rhizosphere bacteria

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基金资助

云南省重大科技专项计划项目(202102AE090005)

云南省科技人才与平台计划项目(202205AF150017)