农药残留快速检测试纸条的研制及应用

Construction and application of paper test strip for rapid detection of pesticide residues

摘要

简单快速地筛查有机磷和氨基甲酸酯类农药残留对食品安全具有重要意义。采用酶抑制法，以重氮固红RC盐为指示剂、乙酸吲哚酚为乙酰胆碱酯酶(AChE)底物，设计开发了一种一步式快速检测试纸条。该试纸条底部为塑料胶板，上面依次粘贴样品垫、酶垫、阻滞垫、底物垫、吸附垫和固定有重氮固红RC盐的硝酸纤维素(NC)膜，通过检测区域颜色的变化可以实现农药残留的定性或定量分析，操作方法简单快捷。不同农药的检出限分别如下:马拉硫磷为0.6 mg·L^-1，氧化乐果为0.4 mg·L^-1，乙酰甲胺磷为1.3 mg·L^-1，敌敌畏为0.1 mg·L^-1，呋喃丹为0.1 mg·L^-1，涕灭威为0.07 mg·L^-1，且在实际果蔬样品检测中显示出良好的重现性和高灵敏度。

Abstract

Simple and rapid screening of organophosphorus and carbamate pesticide residues is of great significance for food safety. In this study, the authors designed and developed a one-step rapid test strip using enzyme inhibition method, with the diazo red RC salt as indicator and indoxyl acetate as substrate of acetylcholinesterase (AChE). The bottom of the test strip is a plastic rubber plate, which is successively pasted with sample pad, enzyme pad, block pad, substrate pad, adsorption pad and nitrocellulose (NC) film that fixed with diazo red RC salt. Qualitative or quantitative analysis of pesticide residues can be achieved by detecting changes in the color of the detection area, and the operation method is simple and fast. The detection limits of different pesticides are as follows: 0.6 mg·L^-1 for malathion, 0.4 mg·L^-1 for omethoate, 1.3 mg·L^-1 for acephate, 0.1 mg·L^-1 for dichlorvos, 0.1 mg·L^-1 for carbofuran, 0.07 mg·L^-1 for aldicarb. It shows good reproducibility and high sensitivity in actual fruit and vegetable samples.

关键词

乙酰胆碱酯酶 / 农药残留 / 快速检测 / 试纸条

Key words

Acetylcholinesterase / Pesticide residue / Rapid detection / Test strip

参考文献

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基金资助

国家自然科学基金(81970173)