镉对广蜜二号南瓜幼苗生长与镉累积特性的影响

Effects of cadmium on the growth and cadmium accumulation characteristics of Guangmi 2 miben pumpkin seedlings

摘要

镉(Cd)是一种毒性强且分布广的重金属污染物，不仅影响植物生长发育，而且危害人类健康。为了更好地 了解 Cd 在南瓜中的迁移规律，深入探究 Cd 对植物的毒害机制和植物的防御反应，将广蜜二号南瓜在 4 种不同质 量浓度(1、3、5、7 mg·L^-1)的 CdSO4·8H2O 溶液中培养 28 d, 对其生长指标、抗氧化酶活性、Cd 吸收转运等特征进行 分析。结果表明，随着 Cd 质量浓度升高，Cd 积累量表现为根部高于茎和叶，Cd 胁迫提高了广蜜二号南瓜幼苗叶片 的超氧化物歧化酶、过氧化物酶、过氧化氢酶活性及丙二醛含量。当溶液中 Cd 质量浓度较低(1 mg·L^-1)时，Cd 对广 蜜二号南瓜幼苗生长无抑制作用，而当溶液中 Cd 质量浓度≥3 mg·L^-1时，对南瓜幼苗的生长抑制程度加重。

Abstract

Cadmium (Cd), a highly toxic and widely distributed heavy metal pollutant, not only affects plant growth and development, but also endangers human health. In order to better understand the migration rule of cadmium in pumpkin, and deeply explore the toxic mechanism of cadmium to plants and the defense response of plants, in this study, Guangmi 2 Miben pumpkin was planted in 4 different concentrations (1, 3, 5, 7 mg·L^-1) of CdSO4·8H2O solution for 28 days, and its growth parameters, antioxidant enzyme activity, cadmium absorption and transport characteristics were analyzed. With the increase of cadmium concentration, the accumulation of cadmium was higher in roots than in stems and leaves, and the activity of superoxide dismutase, peroxidase and catalase and the content of malondialdehyde in leaves of Guangmi 2 Miben pumpkin were increased under cadmium stress. When the concentration of Cd in the solution is low (1 mg·L^-1), Cd has no inhibitory effect on the growth of Guangmi 2, while the concentration of Cd in the solution is above 3 mg·L^-1, the growth inhibition degree of pumpkin is increased with the increase of the concentration of cadmium in the culture solution.

关键词

广蜜二号 / 镉 / 幼苗生长 / 生理特性

Key words

Guangmi 2 / Cadmium / Seedling growth / Physiological characteristics

参考文献

[1] 中华人民共和国环境保护部和国土资源部. 全国土壤污染状况调查公报[R]. (2014-04-17)[2023-11-20]. https://www.mee.gov.cn/gkml/sthjbgw/qt/201404/t20140417_270670.htm.

[2] 王永平, 杨万荣, 廖芳芳, 等. 镉低积累作物筛选及其与超富集植物间套作应用进展[J]. 广东农业科学, 2015, 42(24): 92-98.

[3] 李富荣, 赵洁, 文典, 等. 不同种植模式对土壤重金属铅、镉形态分布的影响[J]. 广东农业科学, 2015, 42(9): 56-61.

[4] 朱彤. 土壤重金属污染现状及检测技术研究[J]. 化工设计通讯, 2022, 48(6): 200-202.

[5] 张猛. 农田中镉的环境行为和影响因素分析[J]. 农业与技术, 2022, 42(12): 112-115.

[6] SANGA T R, MWAKALAPA E B, KALUGENDO K, et al. Effects of cadmium uptake on growth and productivity of Chinese cabbage (Brassica rapa subsp. pekinensis) and pumpkin (Cucurbita moschata Duchesne) vegetables[J]. Journal of Agriculture and Food Research, 2023: 100744.

[7] ALYEMENI M N, AHANGER M A, WIJAYA L, et al. Selenium mitigates cadmium-induced oxidative stress in tomato (Solanum lycopersicum L.) plants by modulating chlorophyll fluorescence, osmolyte accumulation, and antioxidant system[J]. Protoplasma, 2018, 255: 459-469.

[8] 王芳, 杨勇, 张燕, 等. 不同蔬菜对镉的吸收累积及亚细胞分布[J]. 农业环境科学学报, 2009, 28(1): 44-48.

[9] 邓刚, 王刚, 孙梦飞, 等. 镉胁迫下不同水稻品种镉的累积与分布差异[J]. 浙江农业科学, 2016, 57(4): 468-471.

[10] HAN T, SUN M Y, ZHAO J J, et al. The roles of cadmium on growth of seedlings by analysing the composition of metabolites in pumpkin tissues[J]. Ecotoxicology and Environmental Safety, 2021, 226: 112817.

[11] MORENO-CASELLES J, MORAL R, PÉREZ-ESPINOSA A, et al. Cadmium accumulation and distribution in cucumber plant[J]. Journal of Plant Nutrition, 2000, 23(2): 243-250.

[12] BALDANTONI D, MORRA L, ZACCARDELLI M, et al. Cadmium accumulation in leaves of leafy vegetables[J]. Ecotoxicology and Environmental Safety, 2015, 123: 89-94.

[13] 林子江, 贾燕, 夏迪, 等. 土壤调理剂对蔬菜镉吸收的影响研究[J]. 广东农业科学, 2018, 45(5): 47-51.

[14] WANG G, SU M Y, CHEN Y H, et al. Transfer characteristics of cadmium and lead from soil to the edible parts of six vegetable species in southeastern China[J]. Environmental Pollution, 2006, 144(1): 127-135.

[15] 陈碧华, 李庆飞, 周俊国, 等. 9 份中国南瓜幼苗对 Cd 2+的积累特征[J]. 中国瓜菜, 2018, 31(5): 11-15.

[16] 邢乃林, 严蕾艳, 王毓洪, 等. 不同类型砧木嫁接对西瓜果实品质和镉含量的影响[J]. 江西农业学报, 2022, 34(8): 17-21.

[17] 李阳. 镉胁迫下生物炭对南瓜幼苗生长发育及生理生化的影响[D]. 河南新乡: 河南科技学院, 2020.

[18] CAKMAK I, MARSCHNER H. Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves[J]. Plant Resilience, 1992, 98(4): 1222-1227.

[19] PATRA H K, KAR M, MISHRA D . 1978. Catalase activity in leaves and cotyledons during plant development and senescence[J]. Sustainable Plant Production, 1987, 84(2): 450-455.

[20] HODGES D M, DELONG J M, FORNEY C F, et al. Improving the thiobarbituric acid- reactivesubstances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds[J]. Planta, 1999, 207: 604-611.

[21] 杨和连, 张煜广, 郭卫丽, 等. 南瓜砧木嫁接对黄瓜幼苗生长及镉积累特性的影响[J]. 中国蔬菜, 2022, (6): 79-83.

[22] 李阳, 韩涛, 常艺红, 等. 南瓜、黄瓜和油菜幼苗对镉胁迫的响应[J]. 中国瓜菜, 2020, 33(7): 28-33.

[23] 李贞霞, 任秀娟, 孙涌栋. 南瓜对镉的吸收积累特性研究[J]. 生态科学, 2006, 25(5): 423-425.

[24] LYSENKO E A, KLAUS A A, PSHYBYTKO N L, et al. Cadmium accumulation in chloroplasts and its impact on chloroplastic processes in barley and maize[J]. Photosynthesis Research, 2015, 125(1/2): 291-303.

[25] HÉDIJI H, DJEBALI W, BELKADHI A, et al. Impact of long-term cadmium exposure on mineral content of Solanum lycopersicum plants:Consequences on fruit production[J]. South African Journal of Botany, 2015, 97: 176-181.

[26] BAILEY-SERRES J, MITTLER R. The roles of reactive oxygen species in plant cells[J]. Plant Physiology, 2006, 141(2): 311.

[27] 孙园园, 李娅迪, 江辉, 等. 镉胁迫对 3 种草本植物种子萌发及幼苗初期抗氧化酶活性的影响[J]. 广东农业科学, 2014, 41(22): 35-38.

[28] HALL J L. Cellular mechanisms for heavy metal detoxification and tolerance[J]. Journal of Experimental Botany, 2002, 53(366): 1-11.

基金资助

广东省农业科学院食品营养与健康研究中心建设运行经费(XTXM 202205)

广西科技计划项目(桂科AB23026076)