大白菜根肿病抗感品种间的侵染过程及生理生化差异分析

Infection process and physiological and biochemical difference between clubroot-resistant and-susceptible Chinese cabbage lines

摘要

根肿病是由芸薹根肿菌专性侵染十字花科蔬菜作物引起的一种世界性土传病害，对大白菜等蔬菜造成了严重的经济损失。以抗根肿病大白菜 DH 系(DH40R)和感病大白菜 DH 系(DH199S)为试验材料，通过观察根肿菌侵染的差异、防御酶活性、MDA 和可溶性蛋白含量的测定研究大白菜根肿病抗性机制。结果表明，DH40R 和 DH199S 的根毛侵染发生在接种后第 2 天，随着侵染时间的延长，出现皮层侵染，但 DH40R 的皮层侵染在接菌第 8 天后被阻断，对根肿病具有完全的抗性，而 DH199S 在接菌 8 d 后，根部有肿瘤产生。在接菌后 2、8 和 13 d, 分别对抗、感材料接菌和未接菌根部的防御酶活性、MDA 和可溶性蛋白含量进行测定，发现 DH40R 在这 3 个时间点接菌后 SOD 的活性均显著高于未接菌对照组，在第 8 天达到最高峰值，显著高于 DH199S；DH40R 接菌后的 CAT 活性在第 8 天达到最高峰值 1 572.68 μmol·mL^-1·min^-1，显著高于 DH40R 未接菌对照组和接菌及未接菌处理的 DH199S；在接菌后 8 和 13 d, 抗根肿病大白菜 DH 系 DH40R 的 MDA 含量显著低于接菌后的感病 DH 系 DH199S；接菌后 DH40R 的可溶性蛋白含量在接菌后 8 和 13 d 均显著高于 DH199S。研究结果可为后期解析大白菜根肿病抗性相关机制的研究奠定基础。

Abstract

Clubroot is a worldwide soil-borne disease of the cruciferous plants caused by Plasmodiophora brassicae, leading to serious economic losses to cabbage and other Brassica crops. In order to clarify the clubroot resistance mechanism of Brassica rapa, this study was conducted to detect the differences of infection process, defense enzyme activity and the content of MDA and soluble protein between clubroot-resistant (DH40R) and clubroot-susceptible (DH199S) Chinese cabbage lines. The results showed that the primary (root hair infection) stage was at 2 day after inoculation (dai) in both clubroot-resistant (DH40R) and clubroot-susceptible (DH199S) Chinese cabbage lines. With the extension of the infection time, cortical infection was blocked and showed complete P. brassicae resistance in DH40R, while in DH199S, swollen root occurred in the root after 8 dai. The defense enzyme activity and the content of MDA and soluble protein were measured at 2, 8 and 13 dai. The activity of SOD in DH40R after inoculation is significantly higher than the mock at the whole experimental stage, the highest peak was reached at 8 dai, which was significantly higher than that of DH199S. The CAT activity of DH40R after inoculation reached the highest peak 1 572.68 μmol·mL^-1·min^-1 at 8 dai, which was significantly higher than the mock of DH40R and the inoculated and uninoculated DH199S. The MDA content of DH40R was significantly lower than that of DH199S at 8 and 13 dai. The soluble protein content in DH40R was higher than that of DH199S at 8 and 13 dai. Overall, these results can lay foundation for further exploration of resistance mechanism of B. rapa against P. brassicae infection.

关键词

大白菜 / 根肿病 / 侵染过程 / 生理生化指标

Key words

Chinese cabbage / Clubroot / Infection / Physiological and biochemical index

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基金资助

科创中原青年人才托举工程(2023HYTP022)

河南省农业科学院优秀青年基金(2024YQ12)

河南省优势学科联合基金重点项目(232301420024)

河南省重点科技攻关(232102111105)

河南省农业科学院创新团队(2023TD06)