钙镁浓度变化对藻类生长及藻源有机物化学特征的影响

Effects of calcium and magnesium concentration changes on algal growth and the chemical characteristics of algal-derived organic matter

摘要

喀斯特地区藻华爆发期背景下,钙镁离子浓度变化对藻类的藻源有机物(AOM)影响研究有限。本研究采用光谱分析技术,探究钙镁离子浓度变化对藻类生长的影响,以及对细胞外有机物(EOM)和细胞内有机物(IOM)化学特征的作用机制。结果表明,钙镁浓度升高,小球藻和伪鱼腥藻在稳定期(SP)的细胞数量分别下降22.32%和22.46%。UV-vis与3D-EEM表明,钙镁浓度增加,促进2种藻AOM中芳香族、蛋白质和腐殖质类物质的分泌,小球藻EOM和IOM蛋白质(AP)的占比分别提高至37%和19%;而伪鱼腥藻EOM和IOM中腐殖质(HA)的占比高达61%和60%。FTIR结果显示,位于3 300～3 500 cm^-1的O-H/N-H的官能团,参与了对钙镁的结合。XPS结果证实,2种藻IOM和EOM的C-C/C-H官能团占比均增加,小球藻IOM的C-C/C-H占比提升至72%,而伪鱼腥藻IOM的占比则达到51%。

Abstract

Under the background of algal bloom outbreaks in karst areas,research on the impact of changes in calcium and magnesium concentration on algal organic matter (AOM) is limited.This study uses spectroscopic analysis techniques to investigate the effects of changes in calcium and magnesium concentrations on algal growth, as well as the mechanisms affecting the chemical characteristics of extracellular organic matter (EOM) and intracellular organic matter (IOM).The results showed that with the increase of calcium and magnesium concentration,the cell numbers of Chlorella sp. and Pseudanabaena sp. decreased by 22.32% and 22.46% respectively during the stable phase (SP).UV-vis and 3D-EEM indicate that the increase in calcium and magnesium concentrations promotes the secretion of aromatic,proteinaceous,and humic substances in AOM of both algae.The proportion of proteins (AP) in Chlorella sp. EOM and IOM increased to 37% and 19%,respectively; while the proportion of humic substances (HA) in Pseudanabaena sp. EOM and IOM reached 61% and 60%.FTIR results show that the O-H/N-H functional groups at 3 300～3 500 cm^-1 are involved in binding with calcium and magnesium.XPS results confirmed that the proportion of C-C/C-H functional groups in both algal IOM and EOM increased,with the C-C/C-H proportion in Chlorella sp. IOM rising to 72%,while in Pseudanabaena sp. IOM reached 51%.

关键词

AOM / 钙镁离子 / 化学特征 / 藻类响应机制

Key words

algal-derived organic matter / calcium and magnesium ions / chemical characteristics / algal response mechanisms

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基金资助

国家自然科学基金资助项目(42167050、41867048);贵州省科技厅优秀科技青年人才培养计划资助项目(黔科合平台人才-YQK[2023]017)