壮药扁担藤总酚酸类成分含量测定及提取工艺优化

Determination of total phenolic acid content in the zhuang-medicinal plant Tetrastigma planicaule and its optimization of extraction technology

摘要

目的 测定壮药扁担藤(Tetrastigma planicaule )总酚酸含量,并优化其提取工艺。方法 采用微板比色法(酶标仪读数)对扁担藤总酚酸进行含量测定,并以扁担藤总酚酸含量作为关键评价指标,根据单因素试验结果,采用Box-Behnken设计-响应面法对扁担藤总酚酸提取工艺进行优化。结果 扁担藤总酚酸最佳提取工艺条件为:乙醇浓度为45%,乙醇添加量为20倍,提取时间为53 min。在此条件下,得到的扁担藤醇提取物总酚酸含量为1.53%。结论 优选出的提取工艺简单、稳定、可靠,可获得较高含量的总酚酸,可为扁担藤的进一步开发利用提供科学依据。

Abstract

Objective To determine the total phenolic acid content in the Zhuang-medicinal plant Tetrastigma planicaule and optimize its extraction process. Methods A microplate colorimetric assay (enzyme-microplate reader) was employed to quantify the total phenolic acids. Using total phenolic acid content as the critical evaluation indicator, the extration process was optimized by using the Box-Behnken design-response surface method based on single-factor experimental results. Results The optimum extraction conditions were established as follows: 45% (v/v) ethanol, 20-fold solvent-to-material ratio, and 53 min extraction time. Under these conditions, the ethanolic extract contained 1.53% total phenolic acids. Conclusion The optimized protocol is simple, stable and reproducible, affording a high content of total phenolic acids and providing a reliable scientific basis for the further development and utilization of T. planicaule.

关键词

扁担藤 / 总酚酸 / 含量测定 / Box-Behnken设计-响应面法

Key words

Tetrastigma planicaule / total phenolic acids / determination of content / Box-Behnken design-response surface method

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基金资助

国家自然科学基金项目(82060713)